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Production of soluble regulatory hydrogenase from Ralstonia eutropha in Escherichia coli using a fed-batch-based autoinduction system

BACKGROUND: Autoinduction systems can regulate protein production in Escherichia coli without the need to monitor cell growth or add inducer at the proper time following culture growth. Compared to classical IPTG induction, autoinduction provides a simple and fast way to obtain high protein yields....

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Detalles Bibliográficos
Autores principales: Fan, Qin, Neubauer, Peter, Gimpel, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8522226/
https://www.ncbi.nlm.nih.gov/pubmed/34663324
http://dx.doi.org/10.1186/s12934-021-01690-4