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Dysregulation of T(FH)-B-T(RM) lymphocyte cooperation is associated with unfavorable anti-PD-1 responses in EGFR-mutant lung cancer
Patients with non-small cell lung cancer (NSCLC) with epidermal growth factor receptor (EGFR) mutations exhibit an unfavorable response to PD-1 inhibitor through unclear mechanisms. Hypothesizing that EGFR mutations alter tumor-immune interactions, we compare tumor-infiltrating lymphocytes between E...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8523541/ https://www.ncbi.nlm.nih.gov/pubmed/34663810 http://dx.doi.org/10.1038/s41467-021-26362-0 |
Sumario: | Patients with non-small cell lung cancer (NSCLC) with epidermal growth factor receptor (EGFR) mutations exhibit an unfavorable response to PD-1 inhibitor through unclear mechanisms. Hypothesizing that EGFR mutations alter tumor-immune interactions, we compare tumor-infiltrating lymphocytes between EGFR mutant (EGFR-MT) and wild type (EGFR-WT) tumors through single-cell transcriptomic analysis. We find that B cells, CXCL13-producing follicular helper CD4(+) T (T(FH))-like cells, and tissue-resident memory CD8(+) T (T(RM))-like cells decreased in EGFR-MT tumors. The NOTCH-RBPJ regulatory network, which is vital for persistence of T(RM) state, is perturbed, and the interactions between T(FH) and B cells through the CXCL13-CXCR5 axis disappear in EGFR-MT tumors. Notably, the proportion of T(RM)-like cells is predictive for anti-PD-1 response in NSCLC. Our findings suggest that the impairment of T(FH)-B-T(RM) cooperation in tertiary lymphoid structure formation, accompanied by the dysregulation of T(RM) homeostasis and the loss of T(FH)-B crosstalk, underlies unfavorable anti-PD-1 response in EGFR-MT lung tumors. |
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