Targetron-Assisted Delivery of Exogenous DNA Sequences into Pseudomonas putida through CRISPR-Aided Counterselection

[Image: see text] Genome editing methods based on group II introns (known as targetron technology) have long been used as a gene knockout strategy in a wide range of organisms, in a fashion independent of homologous recombination. Yet, their utility as delivery systems has typically been suboptimal...

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Detalles Bibliográficos
Autores principales: Velázquez, Elena, Al-Ramahi, Yamal, Tellechea-Luzardo, Jonathan, Krasnogor, Natalio, de Lorenzo, Víctor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8524655/
https://www.ncbi.nlm.nih.gov/pubmed/34601868
http://dx.doi.org/10.1021/acssynbio.1c00199

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8524655/
https://www.ncbi.nlm.nih.gov/pubmed/34601868
http://dx.doi.org/10.1021/acssynbio.1c00199

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