CRISPR/Cas9 Mutagenesis by Translocation of Cas9 Protein Into Plant Cells via the Agrobacterium Type IV Secretion System

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is a powerful tool for genome engineering in plants. The RNA-guided Cas9 endonuclease is usually delivered into plant cells as a DNA construct encoding Cas9 and the single guide RNA (sgRNA). However...

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Detalles Bibliográficos
Autores principales: Schmitz, Daan J., Ali, Zahir, Wang, Chenglong, Aljedaani, Fatimah, Hooykaas, Paul J. J., Mahfouz, Magdy, de Pater, Sylvia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Genome Editing
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525350/
https://www.ncbi.nlm.nih.gov/pubmed/34713215
http://dx.doi.org/10.3389/fgeed.2020.00006

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525350/
https://www.ncbi.nlm.nih.gov/pubmed/34713215
http://dx.doi.org/10.3389/fgeed.2020.00006

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