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Precise Genome Editing in miRNA Target Site via Gene Targeting and Subsequent Single-Strand-Annealing-Mediated Excision of the Marker Gene in Plants

Gene targeting (GT) enables precise genome modification—e.g., the introduction of base substitutions—using donor DNA as a template. Combined with clean excision of the selection marker used to select GT cells, GT is expected to become a standard, generally applicable, base editing system. Previously...

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Detalles Bibliográficos
Autores principales: Ohtsuki, Namie, Kizawa, Keiko, Mori, Akiko, Nishizawa-Yokoi, Ayako, Komatsuda, Takao, Yoshida, Hitoshi, Hayakawa, Katsuyuki, Toki, Seiichi, Saika, Hiroaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525353/
https://www.ncbi.nlm.nih.gov/pubmed/34713238
http://dx.doi.org/10.3389/fgeed.2020.617713