Enhanced FnCas12a-Mediated Targeted Mutagenesis Using crRNA With Altered Target Length in Rice

The CRISPR/Cas12a (Cpf1) system utilizes a thymidine-rich protospacer adjacent motif (PAM) and generates DNA ends with a 5′ overhang. These properties differ from those of CRISPR/Cas9, making Cas12a an attractive alternative in the CRISPR toolbox. However, genome editing efficiencies of Cas12a ortho...

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Detalles Bibliográficos
Autores principales: Negishi, Katsuya, Mikami, Masafumi, Toki, Seiichi, Endo, Masaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Genome Editing
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525410/
https://www.ncbi.nlm.nih.gov/pubmed/34713233
http://dx.doi.org/10.3389/fgeed.2020.608563

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525410/
https://www.ncbi.nlm.nih.gov/pubmed/34713233
http://dx.doi.org/10.3389/fgeed.2020.608563

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