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Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents

The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized t...

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Detalles Bibliográficos
Autores principales: Haile, Simon, Nikiforuk, Aidan M., Pandoh, Pawan K., Twa, David D.W., Smailus, Duane E., Nguyen, Jason, Pleasance, Stephen, Wong, Angus, Zhao, Yongjun, Eisler, Diane, Moksa, Michelle, Cao, Qi, Wong, Marcus, Su, Edmund, Krzywinski, Martin, Nelson, Jessica, Mungall, Andrew J., Tsang, Frankie, Prentice, Leah M., Jassem, Agatha, Manges, Amee R., Jones, Steven J.M., Coope, Robin J., Prystajecky, Natalie, Marra, Marco A., Krajden, Mel, Hirst, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8527638/
https://www.ncbi.nlm.nih.gov/pubmed/34687784
http://dx.doi.org/10.1016/j.jviromet.2021.114339