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Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents
The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized t...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The Authors. Published by Elsevier B.V.
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8527638/ https://www.ncbi.nlm.nih.gov/pubmed/34687784 http://dx.doi.org/10.1016/j.jviromet.2021.114339 |
| _version_ | 1784586108738207744 |
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| author | Haile, Simon Nikiforuk, Aidan M. Pandoh, Pawan K. Twa, David D.W. Smailus, Duane E. Nguyen, Jason Pleasance, Stephen Wong, Angus Zhao, Yongjun Eisler, Diane Moksa, Michelle Cao, Qi Wong, Marcus Su, Edmund Krzywinski, Martin Nelson, Jessica Mungall, Andrew J. Tsang, Frankie Prentice, Leah M. Jassem, Agatha Manges, Amee R. Jones, Steven J.M. Coope, Robin J. Prystajecky, Natalie Marra, Marco A. Krajden, Mel Hirst, Martin |
| author_facet | Haile, Simon Nikiforuk, Aidan M. Pandoh, Pawan K. Twa, David D.W. Smailus, Duane E. Nguyen, Jason Pleasance, Stephen Wong, Angus Zhao, Yongjun Eisler, Diane Moksa, Michelle Cao, Qi Wong, Marcus Su, Edmund Krzywinski, Martin Nelson, Jessica Mungall, Andrew J. Tsang, Frankie Prentice, Leah M. Jassem, Agatha Manges, Amee R. Jones, Steven J.M. Coope, Robin J. Prystajecky, Natalie Marra, Marco A. Krajden, Mel Hirst, Martin |
| author_sort | Haile, Simon |
| collection | PubMed |
| description | The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity. |
| format | Online Article Text |
| id | pubmed-8527638 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | The Authors. Published by Elsevier B.V. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-85276382021-10-20 Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents Haile, Simon Nikiforuk, Aidan M. Pandoh, Pawan K. Twa, David D.W. Smailus, Duane E. Nguyen, Jason Pleasance, Stephen Wong, Angus Zhao, Yongjun Eisler, Diane Moksa, Michelle Cao, Qi Wong, Marcus Su, Edmund Krzywinski, Martin Nelson, Jessica Mungall, Andrew J. Tsang, Frankie Prentice, Leah M. Jassem, Agatha Manges, Amee R. Jones, Steven J.M. Coope, Robin J. Prystajecky, Natalie Marra, Marco A. Krajden, Mel Hirst, Martin J Virol Methods Short Communication The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity. The Authors. Published by Elsevier B.V. 2022-01 2021-10-20 /pmc/articles/PMC8527638/ /pubmed/34687784 http://dx.doi.org/10.1016/j.jviromet.2021.114339 Text en © 2021 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
| spellingShingle | Short Communication Haile, Simon Nikiforuk, Aidan M. Pandoh, Pawan K. Twa, David D.W. Smailus, Duane E. Nguyen, Jason Pleasance, Stephen Wong, Angus Zhao, Yongjun Eisler, Diane Moksa, Michelle Cao, Qi Wong, Marcus Su, Edmund Krzywinski, Martin Nelson, Jessica Mungall, Andrew J. Tsang, Frankie Prentice, Leah M. Jassem, Agatha Manges, Amee R. Jones, Steven J.M. Coope, Robin J. Prystajecky, Natalie Marra, Marco A. Krajden, Mel Hirst, Martin Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents |
| title | Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents |
| title_full | Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents |
| title_fullStr | Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents |
| title_full_unstemmed | Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents |
| title_short | Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents |
| title_sort | optimization of magnetic bead-based nucleic acid extraction for sars-cov-2 testing using readily available reagents |
| topic | Short Communication |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8527638/ https://www.ncbi.nlm.nih.gov/pubmed/34687784 http://dx.doi.org/10.1016/j.jviromet.2021.114339 |
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