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Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement

To create novel variants for morphological, physiological, and biotic stress tolerance traits, induced mutations were created using Ethyl Methane Sulphonate (EMS) in the background of Samba Mahsuri (BPT 5204), a popular and mega rice variety of India. A population derived from 10, 500 M(1) plants an...

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Autores principales: Potupureddi, Gopi, Balija, Vishalakshi, Ballichatla, Suneel, C. G., Gokulan, Awalellu, Komal, Lekkala, Swathi, Jallipalli, Karteek, M. G., Gayathri, Mohammad, Ershad, M, Milton, Arutla, Srikanth, Burka, Rajender, Gouri Shankar, Laha, Ayyangari Phani, Padmakumari, Lella Venkata, SubbaRao, Raman Meenakshi, Sundaram, B. C., Viraktamath, Vemuri, Ravindra Babu, Brahma, Kranthi, Madnala, Raju, Patel, Hitendra Kumar, Sonti, Ramesh Venkata, Madhav, Maganti Sheshu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528289/
https://www.ncbi.nlm.nih.gov/pubmed/34669755
http://dx.doi.org/10.1371/journal.pone.0258816
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author Potupureddi, Gopi
Balija, Vishalakshi
Ballichatla, Suneel
C. G., Gokulan
Awalellu, Komal
Lekkala, Swathi
Jallipalli, Karteek
M. G., Gayathri
Mohammad, Ershad
M, Milton
Arutla, Srikanth
Burka, Rajender
Gouri Shankar, Laha
Ayyangari Phani, Padmakumari
Lella Venkata, SubbaRao
Raman Meenakshi, Sundaram
B. C., Viraktamath
Vemuri, Ravindra Babu
Brahma, Kranthi
Madnala, Raju
Patel, Hitendra Kumar
Sonti, Ramesh Venkata
Madhav, Maganti Sheshu
author_facet Potupureddi, Gopi
Balija, Vishalakshi
Ballichatla, Suneel
C. G., Gokulan
Awalellu, Komal
Lekkala, Swathi
Jallipalli, Karteek
M. G., Gayathri
Mohammad, Ershad
M, Milton
Arutla, Srikanth
Burka, Rajender
Gouri Shankar, Laha
Ayyangari Phani, Padmakumari
Lella Venkata, SubbaRao
Raman Meenakshi, Sundaram
B. C., Viraktamath
Vemuri, Ravindra Babu
Brahma, Kranthi
Madnala, Raju
Patel, Hitendra Kumar
Sonti, Ramesh Venkata
Madhav, Maganti Sheshu
author_sort Potupureddi, Gopi
collection PubMed
description To create novel variants for morphological, physiological, and biotic stress tolerance traits, induced mutations were created using Ethyl Methane Sulphonate (EMS) in the background of Samba Mahsuri (BPT 5204), a popular and mega rice variety of India. A population derived from 10, 500 M(1) plants and their descendants were phenotyped for a wide range of traits leading to the identification of 124 mutants having variations in key agro-morphological traits, and 106 mutants exhibiting variation for physiological traits. Higher yield is the ultimate goal of crop improvement and we identified 574 mutants having higher yield compared to wild type by having better yield attributing traits. Further, a total of 50 mutants showed better panicle exertion phenotypes as compared to Samba Mahsuri leading to enhancement of yield. Upon rigorous screening for three major biotic stresses, 8 mutants showed enhanced tolerance for yellow stem borer (YSB), and 13 different mutants each showed enhanced tolerance for sheath blight (ShB) and bacterial leaf blight (BLB), respectively. In addition, screening at multiple locations that have diverse field isolates identified 3, 3, and 5 lines for tolerance to ShB, YSB and BLB, respectively. On the whole, 1231 desired mutant lines identified at M(2) were forwarded to an advanced generation (M(5)). PCR based allele mining indicated that the BLB tolerant mutants have a different allele than the reported alleles for well-known genes affecting bacterial blight resistance. Whole genome re-sequencing revealed substantial variation in comparison to Samba Mahsuri. The lines showing enhanced tolerance to important biotic stresses (YSB, ShB and BLB) as well as several economically important traits are unique genetic resources which can be utilized for the identification of novel genes/alleles for different traits. The lines which have better agronomic features can be used as pre-breeding lines. The entire mutant population is maintained as a national resource for genetic improvement of the rice crop.
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spelling pubmed-85282892021-10-21 Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement Potupureddi, Gopi Balija, Vishalakshi Ballichatla, Suneel C. G., Gokulan Awalellu, Komal Lekkala, Swathi Jallipalli, Karteek M. G., Gayathri Mohammad, Ershad M, Milton Arutla, Srikanth Burka, Rajender Gouri Shankar, Laha Ayyangari Phani, Padmakumari Lella Venkata, SubbaRao Raman Meenakshi, Sundaram B. C., Viraktamath Vemuri, Ravindra Babu Brahma, Kranthi Madnala, Raju Patel, Hitendra Kumar Sonti, Ramesh Venkata Madhav, Maganti Sheshu PLoS One Research Article To create novel variants for morphological, physiological, and biotic stress tolerance traits, induced mutations were created using Ethyl Methane Sulphonate (EMS) in the background of Samba Mahsuri (BPT 5204), a popular and mega rice variety of India. A population derived from 10, 500 M(1) plants and their descendants were phenotyped for a wide range of traits leading to the identification of 124 mutants having variations in key agro-morphological traits, and 106 mutants exhibiting variation for physiological traits. Higher yield is the ultimate goal of crop improvement and we identified 574 mutants having higher yield compared to wild type by having better yield attributing traits. Further, a total of 50 mutants showed better panicle exertion phenotypes as compared to Samba Mahsuri leading to enhancement of yield. Upon rigorous screening for three major biotic stresses, 8 mutants showed enhanced tolerance for yellow stem borer (YSB), and 13 different mutants each showed enhanced tolerance for sheath blight (ShB) and bacterial leaf blight (BLB), respectively. In addition, screening at multiple locations that have diverse field isolates identified 3, 3, and 5 lines for tolerance to ShB, YSB and BLB, respectively. On the whole, 1231 desired mutant lines identified at M(2) were forwarded to an advanced generation (M(5)). PCR based allele mining indicated that the BLB tolerant mutants have a different allele than the reported alleles for well-known genes affecting bacterial blight resistance. Whole genome re-sequencing revealed substantial variation in comparison to Samba Mahsuri. The lines showing enhanced tolerance to important biotic stresses (YSB, ShB and BLB) as well as several economically important traits are unique genetic resources which can be utilized for the identification of novel genes/alleles for different traits. The lines which have better agronomic features can be used as pre-breeding lines. The entire mutant population is maintained as a national resource for genetic improvement of the rice crop. Public Library of Science 2021-10-20 /pmc/articles/PMC8528289/ /pubmed/34669755 http://dx.doi.org/10.1371/journal.pone.0258816 Text en © 2021 Potupureddi et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Potupureddi, Gopi
Balija, Vishalakshi
Ballichatla, Suneel
C. G., Gokulan
Awalellu, Komal
Lekkala, Swathi
Jallipalli, Karteek
M. G., Gayathri
Mohammad, Ershad
M, Milton
Arutla, Srikanth
Burka, Rajender
Gouri Shankar, Laha
Ayyangari Phani, Padmakumari
Lella Venkata, SubbaRao
Raman Meenakshi, Sundaram
B. C., Viraktamath
Vemuri, Ravindra Babu
Brahma, Kranthi
Madnala, Raju
Patel, Hitendra Kumar
Sonti, Ramesh Venkata
Madhav, Maganti Sheshu
Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement
title Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement
title_full Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement
title_fullStr Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement
title_full_unstemmed Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement
title_short Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement
title_sort mutation resource of samba mahsuri revealed the presence of high extent of variations among key traits for rice improvement
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528289/
https://www.ncbi.nlm.nih.gov/pubmed/34669755
http://dx.doi.org/10.1371/journal.pone.0258816
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