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Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement
To create novel variants for morphological, physiological, and biotic stress tolerance traits, induced mutations were created using Ethyl Methane Sulphonate (EMS) in the background of Samba Mahsuri (BPT 5204), a popular and mega rice variety of India. A population derived from 10, 500 M(1) plants an...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528289/ https://www.ncbi.nlm.nih.gov/pubmed/34669755 http://dx.doi.org/10.1371/journal.pone.0258816 |
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author | Potupureddi, Gopi Balija, Vishalakshi Ballichatla, Suneel C. G., Gokulan Awalellu, Komal Lekkala, Swathi Jallipalli, Karteek M. G., Gayathri Mohammad, Ershad M, Milton Arutla, Srikanth Burka, Rajender Gouri Shankar, Laha Ayyangari Phani, Padmakumari Lella Venkata, SubbaRao Raman Meenakshi, Sundaram B. C., Viraktamath Vemuri, Ravindra Babu Brahma, Kranthi Madnala, Raju Patel, Hitendra Kumar Sonti, Ramesh Venkata Madhav, Maganti Sheshu |
author_facet | Potupureddi, Gopi Balija, Vishalakshi Ballichatla, Suneel C. G., Gokulan Awalellu, Komal Lekkala, Swathi Jallipalli, Karteek M. G., Gayathri Mohammad, Ershad M, Milton Arutla, Srikanth Burka, Rajender Gouri Shankar, Laha Ayyangari Phani, Padmakumari Lella Venkata, SubbaRao Raman Meenakshi, Sundaram B. C., Viraktamath Vemuri, Ravindra Babu Brahma, Kranthi Madnala, Raju Patel, Hitendra Kumar Sonti, Ramesh Venkata Madhav, Maganti Sheshu |
author_sort | Potupureddi, Gopi |
collection | PubMed |
description | To create novel variants for morphological, physiological, and biotic stress tolerance traits, induced mutations were created using Ethyl Methane Sulphonate (EMS) in the background of Samba Mahsuri (BPT 5204), a popular and mega rice variety of India. A population derived from 10, 500 M(1) plants and their descendants were phenotyped for a wide range of traits leading to the identification of 124 mutants having variations in key agro-morphological traits, and 106 mutants exhibiting variation for physiological traits. Higher yield is the ultimate goal of crop improvement and we identified 574 mutants having higher yield compared to wild type by having better yield attributing traits. Further, a total of 50 mutants showed better panicle exertion phenotypes as compared to Samba Mahsuri leading to enhancement of yield. Upon rigorous screening for three major biotic stresses, 8 mutants showed enhanced tolerance for yellow stem borer (YSB), and 13 different mutants each showed enhanced tolerance for sheath blight (ShB) and bacterial leaf blight (BLB), respectively. In addition, screening at multiple locations that have diverse field isolates identified 3, 3, and 5 lines for tolerance to ShB, YSB and BLB, respectively. On the whole, 1231 desired mutant lines identified at M(2) were forwarded to an advanced generation (M(5)). PCR based allele mining indicated that the BLB tolerant mutants have a different allele than the reported alleles for well-known genes affecting bacterial blight resistance. Whole genome re-sequencing revealed substantial variation in comparison to Samba Mahsuri. The lines showing enhanced tolerance to important biotic stresses (YSB, ShB and BLB) as well as several economically important traits are unique genetic resources which can be utilized for the identification of novel genes/alleles for different traits. The lines which have better agronomic features can be used as pre-breeding lines. The entire mutant population is maintained as a national resource for genetic improvement of the rice crop. |
format | Online Article Text |
id | pubmed-8528289 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-85282892021-10-21 Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement Potupureddi, Gopi Balija, Vishalakshi Ballichatla, Suneel C. G., Gokulan Awalellu, Komal Lekkala, Swathi Jallipalli, Karteek M. G., Gayathri Mohammad, Ershad M, Milton Arutla, Srikanth Burka, Rajender Gouri Shankar, Laha Ayyangari Phani, Padmakumari Lella Venkata, SubbaRao Raman Meenakshi, Sundaram B. C., Viraktamath Vemuri, Ravindra Babu Brahma, Kranthi Madnala, Raju Patel, Hitendra Kumar Sonti, Ramesh Venkata Madhav, Maganti Sheshu PLoS One Research Article To create novel variants for morphological, physiological, and biotic stress tolerance traits, induced mutations were created using Ethyl Methane Sulphonate (EMS) in the background of Samba Mahsuri (BPT 5204), a popular and mega rice variety of India. A population derived from 10, 500 M(1) plants and their descendants were phenotyped for a wide range of traits leading to the identification of 124 mutants having variations in key agro-morphological traits, and 106 mutants exhibiting variation for physiological traits. Higher yield is the ultimate goal of crop improvement and we identified 574 mutants having higher yield compared to wild type by having better yield attributing traits. Further, a total of 50 mutants showed better panicle exertion phenotypes as compared to Samba Mahsuri leading to enhancement of yield. Upon rigorous screening for three major biotic stresses, 8 mutants showed enhanced tolerance for yellow stem borer (YSB), and 13 different mutants each showed enhanced tolerance for sheath blight (ShB) and bacterial leaf blight (BLB), respectively. In addition, screening at multiple locations that have diverse field isolates identified 3, 3, and 5 lines for tolerance to ShB, YSB and BLB, respectively. On the whole, 1231 desired mutant lines identified at M(2) were forwarded to an advanced generation (M(5)). PCR based allele mining indicated that the BLB tolerant mutants have a different allele than the reported alleles for well-known genes affecting bacterial blight resistance. Whole genome re-sequencing revealed substantial variation in comparison to Samba Mahsuri. The lines showing enhanced tolerance to important biotic stresses (YSB, ShB and BLB) as well as several economically important traits are unique genetic resources which can be utilized for the identification of novel genes/alleles for different traits. The lines which have better agronomic features can be used as pre-breeding lines. The entire mutant population is maintained as a national resource for genetic improvement of the rice crop. Public Library of Science 2021-10-20 /pmc/articles/PMC8528289/ /pubmed/34669755 http://dx.doi.org/10.1371/journal.pone.0258816 Text en © 2021 Potupureddi et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Potupureddi, Gopi Balija, Vishalakshi Ballichatla, Suneel C. G., Gokulan Awalellu, Komal Lekkala, Swathi Jallipalli, Karteek M. G., Gayathri Mohammad, Ershad M, Milton Arutla, Srikanth Burka, Rajender Gouri Shankar, Laha Ayyangari Phani, Padmakumari Lella Venkata, SubbaRao Raman Meenakshi, Sundaram B. C., Viraktamath Vemuri, Ravindra Babu Brahma, Kranthi Madnala, Raju Patel, Hitendra Kumar Sonti, Ramesh Venkata Madhav, Maganti Sheshu Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement |
title | Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement |
title_full | Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement |
title_fullStr | Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement |
title_full_unstemmed | Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement |
title_short | Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement |
title_sort | mutation resource of samba mahsuri revealed the presence of high extent of variations among key traits for rice improvement |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528289/ https://www.ncbi.nlm.nih.gov/pubmed/34669755 http://dx.doi.org/10.1371/journal.pone.0258816 |
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