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Effect of organic acids on growth performance, intestinal morphology, and immunity of broiler chickens with and without coccidial challenge

A total of 360-day-old broiler chicks were allocated into six groups in 2 (Coccidial challenge or not) × 3 (dietary treatments) factorial design. Three dietary treatments including: basic diet, basic diet plus organic acids (OAs) in drinking water, and basic diet plus OAs in the feed with and withou...

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Autores principales: Mustafa, Ahsan, Bai, Shiping, Zeng, Qiufeng, Ding, Xuemei, Wang, Jianping, Xuan, Yue, Su, Zhuowei, Zhang, Keying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528927/
https://www.ncbi.nlm.nih.gov/pubmed/34669066
http://dx.doi.org/10.1186/s13568-021-01299-1
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author Mustafa, Ahsan
Bai, Shiping
Zeng, Qiufeng
Ding, Xuemei
Wang, Jianping
Xuan, Yue
Su, Zhuowei
Zhang, Keying
author_facet Mustafa, Ahsan
Bai, Shiping
Zeng, Qiufeng
Ding, Xuemei
Wang, Jianping
Xuan, Yue
Su, Zhuowei
Zhang, Keying
author_sort Mustafa, Ahsan
collection PubMed
description A total of 360-day-old broiler chicks were allocated into six groups in 2 (Coccidial challenge or not) × 3 (dietary treatments) factorial design. Three dietary treatments including: basic diet, basic diet plus organic acids (OAs) in drinking water, and basic diet plus OAs in the feed with and without coccidial challenge. The OAs in water or feed improved (P < 0.01) average body weight (ABW), average body weight gain (ABWG), and feed conversion ratio (FCR) as compared with the control diet during starter, grower, and whole experimental period. Coccidial challenge decreased BW, ABWG, and average feed intake (AFI), as well as resulted in poor FCR during the starter and whole experimental period (P < 0.05). Though there was no interaction between OAs supplementation and coccidial challenge, the OAs supplementation improved the overall performance with and without coccidial challenge birds on 21 d and 35 d. IgG was found higher (P = 0.03) in broilers fed OAs in feed without the coccidial challenge group. On 18 d, OAs supplementation in feed increased TNF-γ (P = 0.006), whereas the coccidial challenge decreases TNF-γ (P = 0.01) and IL-10 (P =  < .0001), and increases IgM (P = 0.03), IgG (P = 0.04) and IgA (P = 0.02). On 29 d, the coccidial challenge increases IgM and IgA. On 18 d, jejunal lesion score was found significantly higher in the coccidial challenged group as compared to OAs supplementation with coccidial challenged groups on 18 d (P < 0.0001) and 29 d (P = 0.03). Crypt depth was higher, and Villus-height to Crypt depth ratio was lower in the coccidial challenge group on 18 and 29 d. The Goblet cells were found higher in the non-coccidial challenge on 18 d. After 18 d, 16S rDNA gene sequence analysis of ileal chyme has shown that coccidial challenge decreases Lactobacillus_reuteri species as compared to the non-challenged group (P = 0.02). After 29, Cyanobacteria abundance reduced (P = 0.014) in the challenged group than the non-challenged group at the phylum level. At the genus level, Lactobacillus (P = 0.036) and unidentified Cyanobacteria (P = 0.01) were found higher in the non-challenged group than the coccidial challenge group. The results indicate that the OAs supplementation showed improved responses in a pattern similar to the non-challenged control group by neutralizing the negative effects of the coccidial challenge.
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spelling pubmed-85289272021-11-04 Effect of organic acids on growth performance, intestinal morphology, and immunity of broiler chickens with and without coccidial challenge Mustafa, Ahsan Bai, Shiping Zeng, Qiufeng Ding, Xuemei Wang, Jianping Xuan, Yue Su, Zhuowei Zhang, Keying AMB Express Original Article A total of 360-day-old broiler chicks were allocated into six groups in 2 (Coccidial challenge or not) × 3 (dietary treatments) factorial design. Three dietary treatments including: basic diet, basic diet plus organic acids (OAs) in drinking water, and basic diet plus OAs in the feed with and without coccidial challenge. The OAs in water or feed improved (P < 0.01) average body weight (ABW), average body weight gain (ABWG), and feed conversion ratio (FCR) as compared with the control diet during starter, grower, and whole experimental period. Coccidial challenge decreased BW, ABWG, and average feed intake (AFI), as well as resulted in poor FCR during the starter and whole experimental period (P < 0.05). Though there was no interaction between OAs supplementation and coccidial challenge, the OAs supplementation improved the overall performance with and without coccidial challenge birds on 21 d and 35 d. IgG was found higher (P = 0.03) in broilers fed OAs in feed without the coccidial challenge group. On 18 d, OAs supplementation in feed increased TNF-γ (P = 0.006), whereas the coccidial challenge decreases TNF-γ (P = 0.01) and IL-10 (P =  < .0001), and increases IgM (P = 0.03), IgG (P = 0.04) and IgA (P = 0.02). On 29 d, the coccidial challenge increases IgM and IgA. On 18 d, jejunal lesion score was found significantly higher in the coccidial challenged group as compared to OAs supplementation with coccidial challenged groups on 18 d (P < 0.0001) and 29 d (P = 0.03). Crypt depth was higher, and Villus-height to Crypt depth ratio was lower in the coccidial challenge group on 18 and 29 d. The Goblet cells were found higher in the non-coccidial challenge on 18 d. After 18 d, 16S rDNA gene sequence analysis of ileal chyme has shown that coccidial challenge decreases Lactobacillus_reuteri species as compared to the non-challenged group (P = 0.02). After 29, Cyanobacteria abundance reduced (P = 0.014) in the challenged group than the non-challenged group at the phylum level. At the genus level, Lactobacillus (P = 0.036) and unidentified Cyanobacteria (P = 0.01) were found higher in the non-challenged group than the coccidial challenge group. The results indicate that the OAs supplementation showed improved responses in a pattern similar to the non-challenged control group by neutralizing the negative effects of the coccidial challenge. Springer Berlin Heidelberg 2021-10-20 /pmc/articles/PMC8528927/ /pubmed/34669066 http://dx.doi.org/10.1186/s13568-021-01299-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Mustafa, Ahsan
Bai, Shiping
Zeng, Qiufeng
Ding, Xuemei
Wang, Jianping
Xuan, Yue
Su, Zhuowei
Zhang, Keying
Effect of organic acids on growth performance, intestinal morphology, and immunity of broiler chickens with and without coccidial challenge
title Effect of organic acids on growth performance, intestinal morphology, and immunity of broiler chickens with and without coccidial challenge
title_full Effect of organic acids on growth performance, intestinal morphology, and immunity of broiler chickens with and without coccidial challenge
title_fullStr Effect of organic acids on growth performance, intestinal morphology, and immunity of broiler chickens with and without coccidial challenge
title_full_unstemmed Effect of organic acids on growth performance, intestinal morphology, and immunity of broiler chickens with and without coccidial challenge
title_short Effect of organic acids on growth performance, intestinal morphology, and immunity of broiler chickens with and without coccidial challenge
title_sort effect of organic acids on growth performance, intestinal morphology, and immunity of broiler chickens with and without coccidial challenge
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528927/
https://www.ncbi.nlm.nih.gov/pubmed/34669066
http://dx.doi.org/10.1186/s13568-021-01299-1
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