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Apoptotic M540 bodies present in human semen interfere with flow cytometry-assisted assessment of sperm DNA fragmentation and oxidation

BACKGROUND: The use of flow cytometry (FC) to evaluate sperm DNA fragmentation via deoxynucleotidyl transferase terminal fluorescein dUTP nick-end labeling (TUNEL) has shown inconsistencies compared with conventional fluorescent microscopic analyses. It has been hypothesized that the observed discre...

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Detalles Bibliográficos
Autores principales: Sadeghi, Niloofar, Tavalaee, Marziyeh, Kiani-Esfahani, Abbas, Moazamian, Aron, Gharagozloo, Parviz, Aitken, Robert J., Drevet, Joël R., Nasr-Esfahani, Mohammad Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8529777/
https://www.ncbi.nlm.nih.gov/pubmed/34670490
http://dx.doi.org/10.1186/s12610-021-00143-7