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Treatment with Autophagy Inducer Trehalose Alleviates Memory and Behavioral Impairments and Neuroinflammatory Brain Processes in db/db Mice

Autophagy attenuation has been found in neurodegenerative diseases, aging, diabetes mellitus, and atherosclerosis. In experimental models of neurodegenerative diseases, the correction of autophagy in the brain reverses neuronal and behavioral deficits and hence seems to be a promising therapy for ne...

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Autores principales: Korolenko, Tatiana A., Dubrovina, Nina I., Ovsyukova, Marina V., Bgatova, Nataliya P., Tenditnik, Michael V., Pupyshev, Alexander B., Akopyan, Anna A., Goncharova, Natalya V., Lin, Chih-Li, Zavjalov, Evgeny L., Tikhonova, Maria A., Amstislavskaya, Tamara G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8533743/
https://www.ncbi.nlm.nih.gov/pubmed/34685538
http://dx.doi.org/10.3390/cells10102557
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author Korolenko, Tatiana A.
Dubrovina, Nina I.
Ovsyukova, Marina V.
Bgatova, Nataliya P.
Tenditnik, Michael V.
Pupyshev, Alexander B.
Akopyan, Anna A.
Goncharova, Natalya V.
Lin, Chih-Li
Zavjalov, Evgeny L.
Tikhonova, Maria A.
Amstislavskaya, Tamara G.
author_facet Korolenko, Tatiana A.
Dubrovina, Nina I.
Ovsyukova, Marina V.
Bgatova, Nataliya P.
Tenditnik, Michael V.
Pupyshev, Alexander B.
Akopyan, Anna A.
Goncharova, Natalya V.
Lin, Chih-Li
Zavjalov, Evgeny L.
Tikhonova, Maria A.
Amstislavskaya, Tamara G.
author_sort Korolenko, Tatiana A.
collection PubMed
description Autophagy attenuation has been found in neurodegenerative diseases, aging, diabetes mellitus, and atherosclerosis. In experimental models of neurodegenerative diseases, the correction of autophagy in the brain reverses neuronal and behavioral deficits and hence seems to be a promising therapy for neuropathologies. Our aim was to study the effect of an autophagy inducer, trehalose, on brain autophagy and behavior in a genetic model of diabetes with signs of neuronal damage (db/db mice). A 2% trehalose solution was administered as drinking water during 24 days of the experiment. Expressions of markers of autophagy (LC3-II), neuroinflammation (IBA1), redox state (NOS), and neuronal density (NeuN) in the brain were assessed by immunohistochemical analysis. For behavioral phenotyping, the open field, elevated plus-maze, tail suspension, pre-pulse inhibition, and passive avoidance tests were used. Trehalose caused a slight reduction in increased blood glucose concentration, considerable autophagy activation, and a decrease in the neuroinflammatory response in the brain along with improvements of exploration, locomotor activity, anxiety, depressive-like behavior, and fear learning and memory in db/db mice. Trehalose exerted some beneficial peripheral and systemic effects and partially reversed behavioral alterations in db/db mice. Thus, trehalose as an inducer of mTOR-independent autophagy is effective at alleviating neuronal and behavioral disturbances accompanying experimental diabetes.
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spelling pubmed-85337432021-10-23 Treatment with Autophagy Inducer Trehalose Alleviates Memory and Behavioral Impairments and Neuroinflammatory Brain Processes in db/db Mice Korolenko, Tatiana A. Dubrovina, Nina I. Ovsyukova, Marina V. Bgatova, Nataliya P. Tenditnik, Michael V. Pupyshev, Alexander B. Akopyan, Anna A. Goncharova, Natalya V. Lin, Chih-Li Zavjalov, Evgeny L. Tikhonova, Maria A. Amstislavskaya, Tamara G. Cells Article Autophagy attenuation has been found in neurodegenerative diseases, aging, diabetes mellitus, and atherosclerosis. In experimental models of neurodegenerative diseases, the correction of autophagy in the brain reverses neuronal and behavioral deficits and hence seems to be a promising therapy for neuropathologies. Our aim was to study the effect of an autophagy inducer, trehalose, on brain autophagy and behavior in a genetic model of diabetes with signs of neuronal damage (db/db mice). A 2% trehalose solution was administered as drinking water during 24 days of the experiment. Expressions of markers of autophagy (LC3-II), neuroinflammation (IBA1), redox state (NOS), and neuronal density (NeuN) in the brain were assessed by immunohistochemical analysis. For behavioral phenotyping, the open field, elevated plus-maze, tail suspension, pre-pulse inhibition, and passive avoidance tests were used. Trehalose caused a slight reduction in increased blood glucose concentration, considerable autophagy activation, and a decrease in the neuroinflammatory response in the brain along with improvements of exploration, locomotor activity, anxiety, depressive-like behavior, and fear learning and memory in db/db mice. Trehalose exerted some beneficial peripheral and systemic effects and partially reversed behavioral alterations in db/db mice. Thus, trehalose as an inducer of mTOR-independent autophagy is effective at alleviating neuronal and behavioral disturbances accompanying experimental diabetes. MDPI 2021-09-27 /pmc/articles/PMC8533743/ /pubmed/34685538 http://dx.doi.org/10.3390/cells10102557 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Korolenko, Tatiana A.
Dubrovina, Nina I.
Ovsyukova, Marina V.
Bgatova, Nataliya P.
Tenditnik, Michael V.
Pupyshev, Alexander B.
Akopyan, Anna A.
Goncharova, Natalya V.
Lin, Chih-Li
Zavjalov, Evgeny L.
Tikhonova, Maria A.
Amstislavskaya, Tamara G.
Treatment with Autophagy Inducer Trehalose Alleviates Memory and Behavioral Impairments and Neuroinflammatory Brain Processes in db/db Mice
title Treatment with Autophagy Inducer Trehalose Alleviates Memory and Behavioral Impairments and Neuroinflammatory Brain Processes in db/db Mice
title_full Treatment with Autophagy Inducer Trehalose Alleviates Memory and Behavioral Impairments and Neuroinflammatory Brain Processes in db/db Mice
title_fullStr Treatment with Autophagy Inducer Trehalose Alleviates Memory and Behavioral Impairments and Neuroinflammatory Brain Processes in db/db Mice
title_full_unstemmed Treatment with Autophagy Inducer Trehalose Alleviates Memory and Behavioral Impairments and Neuroinflammatory Brain Processes in db/db Mice
title_short Treatment with Autophagy Inducer Trehalose Alleviates Memory and Behavioral Impairments and Neuroinflammatory Brain Processes in db/db Mice
title_sort treatment with autophagy inducer trehalose alleviates memory and behavioral impairments and neuroinflammatory brain processes in db/db mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8533743/
https://www.ncbi.nlm.nih.gov/pubmed/34685538
http://dx.doi.org/10.3390/cells10102557
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