Accurate detection and quantification of SARS-CoV-2 genomic and subgenomic mRNAs by ddPCR and meta-transcriptomics analysis

SARS-CoV-2 replication requires the synthesis of a set of structural proteins expressed through discontinuous transcription of ten subgenomic mRNAs (sgmRNAs). Here, we have fine-tuned droplet digital PCR (ddPCR) assays to accurately detect and quantify SARS-CoV-2 genomic ORF1ab and sgmRNAs for the n...

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Detalles Bibliográficos
Autores principales: Oranger, Annarita, Manzari, Caterina, Chiara, Matteo, Notario, Elisabetta, Fosso, Bruno, Parisi, Antonio, Bianco, Angelica, Iacobellis, Michela, d’Avenia, Morena, D’Erchia, Anna Maria, Pesole, Graziano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8536764/
https://www.ncbi.nlm.nih.gov/pubmed/34686777
http://dx.doi.org/10.1038/s42003-021-02748-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8536764/
https://www.ncbi.nlm.nih.gov/pubmed/34686777
http://dx.doi.org/10.1038/s42003-021-02748-0

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