Identification of deubiquitinase inhibitors via high-throughput screening using a fluorogenic ubiquitin-rhodamine assay

Identification of selective deubiquitinase (DUB) inhibitors is critical for probe development to further understand and explore DUB biological function. Here, we detail the optimization and deployment of an in vitro fluorogenic ubiquitin-rhodamine assay to conduct high-throughput screening of a smal...

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Detalles Bibliográficos
Autores principales: Varca, Anthony C., Casalena, Dominick, Auld, Douglas, Buhrlage, Sara J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8536791/
https://www.ncbi.nlm.nih.gov/pubmed/34723213
http://dx.doi.org/10.1016/j.xpro.2021.100896

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8536791/
https://www.ncbi.nlm.nih.gov/pubmed/34723213
http://dx.doi.org/10.1016/j.xpro.2021.100896

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