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Re-Use of Caco-2 Monolayers in Permeability Assays—Validation Regarding Cell Monolayer Integrity

Caco-2 monolayers are a common in vitro model used to evaluate human intestinal absorption. The reference protocol requires 21 days post-seeding to establish a stable and confluent cell monolayer, which is used in a single permeability assay during the period of monolayer stability (up to day 30). I...

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Detalles Bibliográficos
Autores principales: Pires, Cristiana L., Praça, Catarina, Martins, Patrícia A. T., Batista de Carvalho, Ana L. M., Ferreira, Lino, Marques, Maria Paula M., Moreno, Maria João
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537988/
https://www.ncbi.nlm.nih.gov/pubmed/34683857
http://dx.doi.org/10.3390/pharmaceutics13101563