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Evaluation of Tetracycline Resistance and Determination of the Tentative Microbiological Cutoff Values in Lactic Acid Bacterial Species

Lactic acid bacteria (LAB) are widely used as probiotics in the food industry owing to their beneficial effects on human health. However, numerous antibiotic resistance genes have been found in LAB strains, especially tetracycline resistance genes. Notably, the potential transferability of these gen...

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Autores principales: Ma, Qingqing, Pei, Zhangming, Fang, Zhifeng, Wang, Hongchao, Zhu, Jinlin, Lee, Yuan-kun, Zhang, Hao, Zhao, Jianxin, Lu, Wenwei, Chen, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538481/
https://www.ncbi.nlm.nih.gov/pubmed/34683449
http://dx.doi.org/10.3390/microorganisms9102128
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author Ma, Qingqing
Pei, Zhangming
Fang, Zhifeng
Wang, Hongchao
Zhu, Jinlin
Lee, Yuan-kun
Zhang, Hao
Zhao, Jianxin
Lu, Wenwei
Chen, Wei
author_facet Ma, Qingqing
Pei, Zhangming
Fang, Zhifeng
Wang, Hongchao
Zhu, Jinlin
Lee, Yuan-kun
Zhang, Hao
Zhao, Jianxin
Lu, Wenwei
Chen, Wei
author_sort Ma, Qingqing
collection PubMed
description Lactic acid bacteria (LAB) are widely used as probiotics in the food industry owing to their beneficial effects on human health. However, numerous antibiotic resistance genes have been found in LAB strains, especially tetracycline resistance genes. Notably, the potential transferability of these genes poses safety risks. To comprehensively evaluate tetracycline resistance in LAB, we determined the tetracycline susceptibility patterns of 478 LAB strains belonging to four genera and eight species. By comparing phenotypes with genotypes based on genome-wide annotations, five tetracycline resistance genes, tet(M), tet(W/N/W), tet(L), tet(S), and tet(45), were detected in LAB. Multiple LAB strains without tetracycline resistance genes were found to be resistant to tetracycline at the currently recommended cutoff values. Thus, based on the minimum inhibitory concentrations of tetracycline for these LAB strains, the species-specific microbiological cutoff values for Lactobacillus (para)gasseri, Lactobacillus johnsonii, and Lactobacillus crispatus to tetracycline were first developed using the Turnidge, Kronvall, and eyeball methods. The cutoff values for Lactiplantibacillus plantarum were re-established and could be used to better distinguish susceptible strains from strains with acquired resistance. Finally, we verified that these five genes play a role in tetracycline resistance and found that tet(M) and tet(W/N/W) are the most widely distributed tetracycline resistance genes in LAB.
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spelling pubmed-85384812021-10-24 Evaluation of Tetracycline Resistance and Determination of the Tentative Microbiological Cutoff Values in Lactic Acid Bacterial Species Ma, Qingqing Pei, Zhangming Fang, Zhifeng Wang, Hongchao Zhu, Jinlin Lee, Yuan-kun Zhang, Hao Zhao, Jianxin Lu, Wenwei Chen, Wei Microorganisms Article Lactic acid bacteria (LAB) are widely used as probiotics in the food industry owing to their beneficial effects on human health. However, numerous antibiotic resistance genes have been found in LAB strains, especially tetracycline resistance genes. Notably, the potential transferability of these genes poses safety risks. To comprehensively evaluate tetracycline resistance in LAB, we determined the tetracycline susceptibility patterns of 478 LAB strains belonging to four genera and eight species. By comparing phenotypes with genotypes based on genome-wide annotations, five tetracycline resistance genes, tet(M), tet(W/N/W), tet(L), tet(S), and tet(45), were detected in LAB. Multiple LAB strains without tetracycline resistance genes were found to be resistant to tetracycline at the currently recommended cutoff values. Thus, based on the minimum inhibitory concentrations of tetracycline for these LAB strains, the species-specific microbiological cutoff values for Lactobacillus (para)gasseri, Lactobacillus johnsonii, and Lactobacillus crispatus to tetracycline were first developed using the Turnidge, Kronvall, and eyeball methods. The cutoff values for Lactiplantibacillus plantarum were re-established and could be used to better distinguish susceptible strains from strains with acquired resistance. Finally, we verified that these five genes play a role in tetracycline resistance and found that tet(M) and tet(W/N/W) are the most widely distributed tetracycline resistance genes in LAB. MDPI 2021-10-11 /pmc/articles/PMC8538481/ /pubmed/34683449 http://dx.doi.org/10.3390/microorganisms9102128 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ma, Qingqing
Pei, Zhangming
Fang, Zhifeng
Wang, Hongchao
Zhu, Jinlin
Lee, Yuan-kun
Zhang, Hao
Zhao, Jianxin
Lu, Wenwei
Chen, Wei
Evaluation of Tetracycline Resistance and Determination of the Tentative Microbiological Cutoff Values in Lactic Acid Bacterial Species
title Evaluation of Tetracycline Resistance and Determination of the Tentative Microbiological Cutoff Values in Lactic Acid Bacterial Species
title_full Evaluation of Tetracycline Resistance and Determination of the Tentative Microbiological Cutoff Values in Lactic Acid Bacterial Species
title_fullStr Evaluation of Tetracycline Resistance and Determination of the Tentative Microbiological Cutoff Values in Lactic Acid Bacterial Species
title_full_unstemmed Evaluation of Tetracycline Resistance and Determination of the Tentative Microbiological Cutoff Values in Lactic Acid Bacterial Species
title_short Evaluation of Tetracycline Resistance and Determination of the Tentative Microbiological Cutoff Values in Lactic Acid Bacterial Species
title_sort evaluation of tetracycline resistance and determination of the tentative microbiological cutoff values in lactic acid bacterial species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538481/
https://www.ncbi.nlm.nih.gov/pubmed/34683449
http://dx.doi.org/10.3390/microorganisms9102128
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