Evaluation of Gene Polymorphism and Gingival Crevicular Fluid Levels of Matrix Metalloproteinase-3 in a Group of Turkish Periodontitis Patients

Introduction: Periodontitis is characterized by the destruction of tooth-supporting tissues. Matrix metalloproteinases (MMPs) play a significant part in the degradation of collagen structure. The gingival crevicular fluid (GCF) levels of MMPs increase with the progression of periodontal inflammation...

Descripción completa

Detalles Bibliográficos
Autores principales: Kasnak, Gökhan, Yılmaz, Mustafa, Ünsal, Revan Birke Koca, Polat, Nuray Gürel, Fıratlı, Erhan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539123/
https://www.ncbi.nlm.nih.gov/pubmed/34684209
http://dx.doi.org/10.3390/pathogens10101260
_version_ 1784588670636916736
author Kasnak, Gökhan
Yılmaz, Mustafa
Ünsal, Revan Birke Koca
Polat, Nuray Gürel
Fıratlı, Erhan
author_facet Kasnak, Gökhan
Yılmaz, Mustafa
Ünsal, Revan Birke Koca
Polat, Nuray Gürel
Fıratlı, Erhan
author_sort Kasnak, Gökhan
collection PubMed
description Introduction: Periodontitis is characterized by the destruction of tooth-supporting tissues. Matrix metalloproteinases (MMPs) play a significant part in the degradation of collagen structure. The gingival crevicular fluid (GCF) levels of MMPs increase with the progression of periodontal inflammation. Polymorphisms can be responsible for high expression of MMPs and can exacerbate the breakdown of collagen structure. This study aims to investigate the effect of MMP-3 -1171 5A/6A polymorphism and the GCF levels of MMP-3 in a group of Turkish periodontitis patients. Materials and Methods: Non-smoking, stage II grade A periodontitis (S II-Gr A) (n = 68) and stage II grade B periodontitis (S II-Gr C) (n = 64) patients were recruited. Healthy individuals (H) (n = 72) without signs of gingivitis or periodontitis served as the control. Venous blood was collected from participants to obtain DNA, and the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method was used to detect polymorphism. GCF samples were taken to assess MMP-3 levels using an enzyme-linked immunosorbent assay (ELISA). Results: The MMP-3 -1179 5A/6A distribution showed no significant difference between the groups (p > 0.05). However, the MMP-3 GCF levels of the S II-Gr C group were higher than those of both the S II-Gr A and H groups (p < 0.05), and elevated MMP-3 levels were detected in S II-Gr A compared to H (p < 0.05). Conclusion: The MMP-3 GCF levels showed an association with periodontal tissue destruction, although single nucleotide polymorphism was not associated with the S II-Gr C and S II-Gr A groups in the Turkish population.
format Online
Article
Text
id pubmed-8539123
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85391232021-10-24 Evaluation of Gene Polymorphism and Gingival Crevicular Fluid Levels of Matrix Metalloproteinase-3 in a Group of Turkish Periodontitis Patients Kasnak, Gökhan Yılmaz, Mustafa Ünsal, Revan Birke Koca Polat, Nuray Gürel Fıratlı, Erhan Pathogens Article Introduction: Periodontitis is characterized by the destruction of tooth-supporting tissues. Matrix metalloproteinases (MMPs) play a significant part in the degradation of collagen structure. The gingival crevicular fluid (GCF) levels of MMPs increase with the progression of periodontal inflammation. Polymorphisms can be responsible for high expression of MMPs and can exacerbate the breakdown of collagen structure. This study aims to investigate the effect of MMP-3 -1171 5A/6A polymorphism and the GCF levels of MMP-3 in a group of Turkish periodontitis patients. Materials and Methods: Non-smoking, stage II grade A periodontitis (S II-Gr A) (n = 68) and stage II grade B periodontitis (S II-Gr C) (n = 64) patients were recruited. Healthy individuals (H) (n = 72) without signs of gingivitis or periodontitis served as the control. Venous blood was collected from participants to obtain DNA, and the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method was used to detect polymorphism. GCF samples were taken to assess MMP-3 levels using an enzyme-linked immunosorbent assay (ELISA). Results: The MMP-3 -1179 5A/6A distribution showed no significant difference between the groups (p > 0.05). However, the MMP-3 GCF levels of the S II-Gr C group were higher than those of both the S II-Gr A and H groups (p < 0.05), and elevated MMP-3 levels were detected in S II-Gr A compared to H (p < 0.05). Conclusion: The MMP-3 GCF levels showed an association with periodontal tissue destruction, although single nucleotide polymorphism was not associated with the S II-Gr C and S II-Gr A groups in the Turkish population. MDPI 2021-09-29 /pmc/articles/PMC8539123/ /pubmed/34684209 http://dx.doi.org/10.3390/pathogens10101260 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kasnak, Gökhan
Yılmaz, Mustafa
Ünsal, Revan Birke Koca
Polat, Nuray Gürel
Fıratlı, Erhan
Evaluation of Gene Polymorphism and Gingival Crevicular Fluid Levels of Matrix Metalloproteinase-3 in a Group of Turkish Periodontitis Patients
title Evaluation of Gene Polymorphism and Gingival Crevicular Fluid Levels of Matrix Metalloproteinase-3 in a Group of Turkish Periodontitis Patients
title_full Evaluation of Gene Polymorphism and Gingival Crevicular Fluid Levels of Matrix Metalloproteinase-3 in a Group of Turkish Periodontitis Patients
title_fullStr Evaluation of Gene Polymorphism and Gingival Crevicular Fluid Levels of Matrix Metalloproteinase-3 in a Group of Turkish Periodontitis Patients
title_full_unstemmed Evaluation of Gene Polymorphism and Gingival Crevicular Fluid Levels of Matrix Metalloproteinase-3 in a Group of Turkish Periodontitis Patients
title_short Evaluation of Gene Polymorphism and Gingival Crevicular Fluid Levels of Matrix Metalloproteinase-3 in a Group of Turkish Periodontitis Patients
title_sort evaluation of gene polymorphism and gingival crevicular fluid levels of matrix metalloproteinase-3 in a group of turkish periodontitis patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539123/
https://www.ncbi.nlm.nih.gov/pubmed/34684209
http://dx.doi.org/10.3390/pathogens10101260
work_keys_str_mv AT kasnakgokhan evaluationofgenepolymorphismandgingivalcrevicularfluidlevelsofmatrixmetalloproteinase3inagroupofturkishperiodontitispatients
AT yılmazmustafa evaluationofgenepolymorphismandgingivalcrevicularfluidlevelsofmatrixmetalloproteinase3inagroupofturkishperiodontitispatients
AT unsalrevanbirkekoca evaluationofgenepolymorphismandgingivalcrevicularfluidlevelsofmatrixmetalloproteinase3inagroupofturkishperiodontitispatients
AT polatnuraygurel evaluationofgenepolymorphismandgingivalcrevicularfluidlevelsofmatrixmetalloproteinase3inagroupofturkishperiodontitispatients
AT fıratlıerhan evaluationofgenepolymorphismandgingivalcrevicularfluidlevelsofmatrixmetalloproteinase3inagroupofturkishperiodontitispatients