Photoactivatable nanoCRISPR/Cas9 System Based on crRNA Reversibly Immobilized on Carbon Nanoparticles

Here, we proposed a new approach to engineering a photoactivatable CRISPR/Cas9 gene-editing system. The novel nanoCRISPR/Cas9 system is based on the use of auxiliary photocleavable oligodeoxyribonucleotides (PC-DNAs) complementary to crRNA. PC-DNAs contained up to three UV-sensitive linkers made of...

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Detalles Bibliográficos
Autores principales: Semikolenova, Olga, Sakovina, Lubov, Akhmetova, Elizaveta, Kim, Daria, Vokhtantsev, Ivan, Golyshev, Victor, Vorobyeva, Mariya, Novopashin, Sergey, Novopashina, Darya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539621/
https://www.ncbi.nlm.nih.gov/pubmed/34681578
http://dx.doi.org/10.3390/ijms222010919

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539621/
https://www.ncbi.nlm.nih.gov/pubmed/34681578
http://dx.doi.org/10.3390/ijms222010919

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