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Expansion of CRISPR Targeting Sites Using an Integrated Gene-Editing System in Apis mellifera

SIMPLE SUMMARY: CRISPR/Cas9, a versatile gene manipulation tool, has been harnessed for targeted genome engineering in honeybees. However, until now, only SpCas9 that enables NGG recognition has been shown to manipulate the genome in A. mellifera, limiting the editable range to the NGG-included loci...

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Detalles Bibliográficos
Autores principales: Liang, Liqiang, Li, Zhenghanqing, Li, Qiufang, Wang, Xiuxiu, Su, Songkun, Nie, Hongyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540347/
https://www.ncbi.nlm.nih.gov/pubmed/34680723
http://dx.doi.org/10.3390/insects12100954