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Expansion of CRISPR Targeting Sites Using an Integrated Gene-Editing System in Apis mellifera
SIMPLE SUMMARY: CRISPR/Cas9, a versatile gene manipulation tool, has been harnessed for targeted genome engineering in honeybees. However, until now, only SpCas9 that enables NGG recognition has been shown to manipulate the genome in A. mellifera, limiting the editable range to the NGG-included loci...
Autores principales: | Liang, Liqiang, Li, Zhenghanqing, Li, Qiufang, Wang, Xiuxiu, Su, Songkun, Nie, Hongyi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540347/ https://www.ncbi.nlm.nih.gov/pubmed/34680723 http://dx.doi.org/10.3390/insects12100954 |
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