Phenotypic Plasticity of Common Wasps in an Industrially Polluted Environment in Southwestern Finland

SIMPLE SUMMARY: Social insects are ecologically and economically important as ecosystem engineers, agricultural pest predators, pollinators, and seed dispersers. Many of the vespid wasps are social insects. Our study species, Common wasp Vespula vulgaris, is native to Finland and classified as invasive in some other parts of the world. The Common wasp have conspicuous yellow and black pigmentation. Their functions and activities in the environment expose the species to environmental pollutants and this study assessed the effect of heavy-metals on common wasps collected from the vicinity of a metal smelter in southwestern Finland. The samples collected were analyzed using various methods such as color morph categorization, electron microscopy, metal analysis, and energy dispersive X-ray analysis (EDX). The methods were used to understand the effects of metal pollution on the species and the adaptive response. Our results indicated phenotypic variation between common wasp samples across the pollution gradient and an adaptive melanin encapsulation process. ABSTRACT: Insects vary in the degree of their adaptability to environmental contamination. Determining the responses with phenotypic plasticity in ecologically important species in polluted environments will ease further conservation and control actions. Here, we investigated morphological characteristics such as body size, body mass, and color of the common wasp Vespula vulgaris in an industrially polluted environment, considering different levels of metal pollution, and we studied the localization of contaminants in the guts of wasps. We revealed some differences in morphological characteristics and melanization of wasps collected in habitats with high, moderate, and low levels of pollution. The results indicated that V. vulgaris from highly polluted environments had reduced melanin pigmentation on the face but increased melanin pigmentation on the 2nd tergite of the abdomen. In addition, with transmission electron microscopy (TEM) and energy dispersive X-ray analysis (EDX), we found metal particles from the midgut of wasps originating from the polluted environment. Most of the particles were encapsulated with melanin pigment. This finding confirmed that in wasps, ingested metal particles are accumulated in guts and covered by melanin layers. Our data suggest that wasps can tolerate metal contamination but respond phenotypically with modification of their size, coloration, and probably with the directions of the melanin investments (immunity or coloration). Thus, in industrially polluted areas, wasps might probably survive by engaging phenotypic plasticity with no significant or visible impact on the population.