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Comparison of CRISPR–Cas9 Tools for Transcriptional Repression and Gene Disruption in the BEVS

The generation of knock-out viruses using recombineering of bacmids has greatly accelerated scrutiny of baculovirus genes for a variety of applications. However, the CRISPR–Cas9 system is a powerful tool that simplifies sequence-specific genome editing and effective transcriptional regulation of gen...

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Detalles Bibliográficos
Autores principales: Bruder, Mark R., Walji, Sadru-Dean, Aucoin, Marc G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541072/
https://www.ncbi.nlm.nih.gov/pubmed/34696355
http://dx.doi.org/10.3390/v13101925