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CRISPR/Cas9-mediated genome editing directed by a 5S rRNA–tRNA(Gly) hybrid promoter in the thermophilic filamentous fungus Humicola insolens
BACKGROUND: Humicola insolens is a filamentous fungus with high potential of producing neutral and heat- and alkali-resistant cellulase. However, the genetic engineering tools, particularly the genome-editing tool, are scarce, hindering the study of cellulase expression regulation in this organism....
| Autores principales: | , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8542335/ https://www.ncbi.nlm.nih.gov/pubmed/34688310 http://dx.doi.org/10.1186/s13068-021-02057-y |