A genetically encoded sensor for measuring serotonin dynamics

Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded GPCR-Activation-Based 5-HT (GRAB(5-HT)) sensor with high sensitivity, selectivity, subsecond kinetics...

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Autores principales: Wan, Jinxia, Peng, Wanling, Li, Xuelin, Qian, Tongrui, Song, Kun, Zeng, Jianzhi, Deng, Fei, Hao, Suyu, Feng, Jiesi, Zhang, Peng, Zhang, Yajun, Zou, Jing, Pan, Sunlei, Shin, Mimi, Venton, B. Jill, Zhu, J. Julius, Jing, Miao, Xu, Min, Li, Yulong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8544647/
https://www.ncbi.nlm.nih.gov/pubmed/33821000
http://dx.doi.org/10.1038/s41593-021-00823-7
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author Wan, Jinxia
Peng, Wanling
Li, Xuelin
Qian, Tongrui
Song, Kun
Zeng, Jianzhi
Deng, Fei
Hao, Suyu
Feng, Jiesi
Zhang, Peng
Zhang, Yajun
Zou, Jing
Pan, Sunlei
Shin, Mimi
Venton, B. Jill
Zhu, J. Julius
Jing, Miao
Xu, Min
Li, Yulong
author_facet Wan, Jinxia
Peng, Wanling
Li, Xuelin
Qian, Tongrui
Song, Kun
Zeng, Jianzhi
Deng, Fei
Hao, Suyu
Feng, Jiesi
Zhang, Peng
Zhang, Yajun
Zou, Jing
Pan, Sunlei
Shin, Mimi
Venton, B. Jill
Zhu, J. Julius
Jing, Miao
Xu, Min
Li, Yulong
author_sort Wan, Jinxia
collection PubMed
description Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded GPCR-Activation-Based 5-HT (GRAB(5-HT)) sensor with high sensitivity, selectivity, subsecond kinetics, and subcellular resolution. GRAB(5-HT) detects 5-HT release in multiple physiological and pathological conditions in both flies and mice, and provides new insights into the dynamics and mechanisms of 5-HT signaling.
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spelling pubmed-85446472021-10-25 A genetically encoded sensor for measuring serotonin dynamics Wan, Jinxia Peng, Wanling Li, Xuelin Qian, Tongrui Song, Kun Zeng, Jianzhi Deng, Fei Hao, Suyu Feng, Jiesi Zhang, Peng Zhang, Yajun Zou, Jing Pan, Sunlei Shin, Mimi Venton, B. Jill Zhu, J. Julius Jing, Miao Xu, Min Li, Yulong Nat Neurosci Article Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded GPCR-Activation-Based 5-HT (GRAB(5-HT)) sensor with high sensitivity, selectivity, subsecond kinetics, and subcellular resolution. GRAB(5-HT) detects 5-HT release in multiple physiological and pathological conditions in both flies and mice, and provides new insights into the dynamics and mechanisms of 5-HT signaling. 2021-04-05 2021-05 /pmc/articles/PMC8544647/ /pubmed/33821000 http://dx.doi.org/10.1038/s41593-021-00823-7 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Wan, Jinxia
Peng, Wanling
Li, Xuelin
Qian, Tongrui
Song, Kun
Zeng, Jianzhi
Deng, Fei
Hao, Suyu
Feng, Jiesi
Zhang, Peng
Zhang, Yajun
Zou, Jing
Pan, Sunlei
Shin, Mimi
Venton, B. Jill
Zhu, J. Julius
Jing, Miao
Xu, Min
Li, Yulong
A genetically encoded sensor for measuring serotonin dynamics
title A genetically encoded sensor for measuring serotonin dynamics
title_full A genetically encoded sensor for measuring serotonin dynamics
title_fullStr A genetically encoded sensor for measuring serotonin dynamics
title_full_unstemmed A genetically encoded sensor for measuring serotonin dynamics
title_short A genetically encoded sensor for measuring serotonin dynamics
title_sort genetically encoded sensor for measuring serotonin dynamics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8544647/
https://www.ncbi.nlm.nih.gov/pubmed/33821000
http://dx.doi.org/10.1038/s41593-021-00823-7
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