Optimized protocol to create deletion in adherent cell lines using CRISPR/Cas9 system

The CRISPR/Cas9 system is a powerful tool for genome editing and is adaptable for a wide range of applications. Here, we have put together a step-by-step protocol for generating knockout cell lines (coding or non-coding region) using CRISPR/Cas9 tool. The protocol below has been tested on adherent c...

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Detalles Bibliográficos
Autores principales: Farooq, Umer, Notani, Dimple
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551220/
https://www.ncbi.nlm.nih.gov/pubmed/34746853
http://dx.doi.org/10.1016/j.xpro.2021.100857

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551220/
https://www.ncbi.nlm.nih.gov/pubmed/34746853
http://dx.doi.org/10.1016/j.xpro.2021.100857

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