Optimized protocol to create deletion in adherent cell lines using CRISPR/Cas9 system

The CRISPR/Cas9 system is a powerful tool for genome editing and is adaptable for a wide range of applications. Here, we have put together a step-by-step protocol for generating knockout cell lines (coding or non-coding region) using CRISPR/Cas9 tool. The protocol below has been tested on adherent c...

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Detalles Bibliográficos
Autores principales: Farooq, Umer, Notani, Dimple
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551220/
https://www.ncbi.nlm.nih.gov/pubmed/34746853
http://dx.doi.org/10.1016/j.xpro.2021.100857
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author Farooq, Umer
Notani, Dimple
author_facet Farooq, Umer
Notani, Dimple
author_sort Farooq, Umer
collection PubMed
description The CRISPR/Cas9 system is a powerful tool for genome editing and is adaptable for a wide range of applications. Here, we have put together a step-by-step protocol for generating knockout cell lines (coding or non-coding region) using CRISPR/Cas9 tool. The protocol below has been tested on adherent cell lines such as HeLa and MCF7. However, it may easily be adapted to other adherent cell lines with minor variations. For complete details on the use and execution of this protocol, please refer to Farooq et al. (2021).
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spelling pubmed-85512202021-11-04 Optimized protocol to create deletion in adherent cell lines using CRISPR/Cas9 system Farooq, Umer Notani, Dimple STAR Protoc Protocol The CRISPR/Cas9 system is a powerful tool for genome editing and is adaptable for a wide range of applications. Here, we have put together a step-by-step protocol for generating knockout cell lines (coding or non-coding region) using CRISPR/Cas9 tool. The protocol below has been tested on adherent cell lines such as HeLa and MCF7. However, it may easily be adapted to other adherent cell lines with minor variations. For complete details on the use and execution of this protocol, please refer to Farooq et al. (2021). Elsevier 2021-10-22 /pmc/articles/PMC8551220/ /pubmed/34746853 http://dx.doi.org/10.1016/j.xpro.2021.100857 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Farooq, Umer
Notani, Dimple
Optimized protocol to create deletion in adherent cell lines using CRISPR/Cas9 system
title Optimized protocol to create deletion in adherent cell lines using CRISPR/Cas9 system
title_full Optimized protocol to create deletion in adherent cell lines using CRISPR/Cas9 system
title_fullStr Optimized protocol to create deletion in adherent cell lines using CRISPR/Cas9 system
title_full_unstemmed Optimized protocol to create deletion in adherent cell lines using CRISPR/Cas9 system
title_short Optimized protocol to create deletion in adherent cell lines using CRISPR/Cas9 system
title_sort optimized protocol to create deletion in adherent cell lines using crispr/cas9 system
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551220/
https://www.ncbi.nlm.nih.gov/pubmed/34746853
http://dx.doi.org/10.1016/j.xpro.2021.100857
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