Isolation of human fetal intestinal cells for single-cell RNA sequencing

The intestine has a large number of cell types. Thus, digestion of pure and viable populations is necessary for downstream techniques including single-cell RNA sequencing. We outline a protocol to isolate both epithelial and non-epithelial cells from human fetal samples at high viability, which was...

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Detalles Bibliográficos
Autores principales: Fawkner-Corbett, David, Gerós, Ana Sousa, Antanaviciute, Agne, Simmons, Alison
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551222/
https://www.ncbi.nlm.nih.gov/pubmed/34746860
http://dx.doi.org/10.1016/j.xpro.2021.100890

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551222/
https://www.ncbi.nlm.nih.gov/pubmed/34746860
http://dx.doi.org/10.1016/j.xpro.2021.100890

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