Isolation of human fetal intestinal cells for single-cell RNA sequencing

The intestine has a large number of cell types. Thus, digestion of pure and viable populations is necessary for downstream techniques including single-cell RNA sequencing. We outline a protocol to isolate both epithelial and non-epithelial cells from human fetal samples at high viability, which was...

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Detalles Bibliográficos
Autores principales: Fawkner-Corbett, David, Gerós, Ana Sousa, Antanaviciute, Agne, Simmons, Alison
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551222/
https://www.ncbi.nlm.nih.gov/pubmed/34746860
http://dx.doi.org/10.1016/j.xpro.2021.100890
Descripción
Sumario:The intestine has a large number of cell types. Thus, digestion of pure and viable populations is necessary for downstream techniques including single-cell RNA sequencing. We outline a protocol to isolate both epithelial and non-epithelial cells from human fetal samples at high viability, which was used to produce a full thickness atlas of intestinal cells across human development. This protocol can also be adapted to adult endoscopy and surgical specimens. For details on the use of this protocol, please refer to Fawkner-Corbett et al. (2021).

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