Isolation of human fetal intestinal cells for single-cell RNA sequencing

The intestine has a large number of cell types. Thus, digestion of pure and viable populations is necessary for downstream techniques including single-cell RNA sequencing. We outline a protocol to isolate both epithelial and non-epithelial cells from human fetal samples at high viability, which was...

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Autores principales: Fawkner-Corbett, David, Gerós, Ana Sousa, Antanaviciute, Agne, Simmons, Alison
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551222/
https://www.ncbi.nlm.nih.gov/pubmed/34746860
http://dx.doi.org/10.1016/j.xpro.2021.100890
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author Fawkner-Corbett, David
Gerós, Ana Sousa
Antanaviciute, Agne
Simmons, Alison
author_facet Fawkner-Corbett, David
Gerós, Ana Sousa
Antanaviciute, Agne
Simmons, Alison
author_sort Fawkner-Corbett, David
collection PubMed
description The intestine has a large number of cell types. Thus, digestion of pure and viable populations is necessary for downstream techniques including single-cell RNA sequencing. We outline a protocol to isolate both epithelial and non-epithelial cells from human fetal samples at high viability, which was used to produce a full thickness atlas of intestinal cells across human development. This protocol can also be adapted to adult endoscopy and surgical specimens. For details on the use of this protocol, please refer to Fawkner-Corbett et al. (2021).
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spelling pubmed-85512222021-11-04 Isolation of human fetal intestinal cells for single-cell RNA sequencing Fawkner-Corbett, David Gerós, Ana Sousa Antanaviciute, Agne Simmons, Alison STAR Protoc Protocol The intestine has a large number of cell types. Thus, digestion of pure and viable populations is necessary for downstream techniques including single-cell RNA sequencing. We outline a protocol to isolate both epithelial and non-epithelial cells from human fetal samples at high viability, which was used to produce a full thickness atlas of intestinal cells across human development. This protocol can also be adapted to adult endoscopy and surgical specimens. For details on the use of this protocol, please refer to Fawkner-Corbett et al. (2021). Elsevier 2021-10-22 /pmc/articles/PMC8551222/ /pubmed/34746860 http://dx.doi.org/10.1016/j.xpro.2021.100890 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Fawkner-Corbett, David
Gerós, Ana Sousa
Antanaviciute, Agne
Simmons, Alison
Isolation of human fetal intestinal cells for single-cell RNA sequencing
title Isolation of human fetal intestinal cells for single-cell RNA sequencing
title_full Isolation of human fetal intestinal cells for single-cell RNA sequencing
title_fullStr Isolation of human fetal intestinal cells for single-cell RNA sequencing
title_full_unstemmed Isolation of human fetal intestinal cells for single-cell RNA sequencing
title_short Isolation of human fetal intestinal cells for single-cell RNA sequencing
title_sort isolation of human fetal intestinal cells for single-cell rna sequencing
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551222/
https://www.ncbi.nlm.nih.gov/pubmed/34746860
http://dx.doi.org/10.1016/j.xpro.2021.100890
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