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Isolation of human fetal intestinal cells for single-cell RNA sequencing

The intestine has a large number of cell types. Thus, digestion of pure and viable populations is necessary for downstream techniques including single-cell RNA sequencing. We outline a protocol to isolate both epithelial and non-epithelial cells from human fetal samples at high viability, which was...

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Detalles Bibliográficos
Autores principales:	Fawkner-Corbett, David, Gerós, Ana Sousa, Antanaviciute, Agne, Simmons, Alison
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551222/ https://www.ncbi.nlm.nih.gov/pubmed/34746860 http://dx.doi.org/10.1016/j.xpro.2021.100890

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