Selective and predicable amine conjugation sites by kinetic characterization under excess reagents

The site selectivity for lysine conjugation on a native protein is difficult to control and characterize. Here, we applied mass spectrometry to examine the conjugation kinetics of Trastuzumab-IgG (Her-IgG) and α-lactalbumin under excess linker concentration ([L](0)) based on the modified Michaelis–M...

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Detalles Bibliográficos
Autores principales: Huang, Wei-Chun, Huang, Li-Juan, Hsu, Liang-Sheng, Huang, Shih-Ting, Lo, Wen-Ting, Wang, Tzu-Fan, Sun, Wei-Ting, Wei, Win-Yin, Lee, Ying-Shuan, Chuang, Shih-Hsien, Lee, Chao-Pin, Chou, Ho-Hsuan, Chen, Shu-Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551328/
https://www.ncbi.nlm.nih.gov/pubmed/34707190
http://dx.doi.org/10.1038/s41598-021-00743-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551328/
https://www.ncbi.nlm.nih.gov/pubmed/34707190
http://dx.doi.org/10.1038/s41598-021-00743-3

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