Cargando…

Selective and predicable amine conjugation sites by kinetic characterization under excess reagents

The site selectivity for lysine conjugation on a native protein is difficult to control and characterize. Here, we applied mass spectrometry to examine the conjugation kinetics of Trastuzumab-IgG (Her-IgG) and α-lactalbumin under excess linker concentration ([L](0)) based on the modified Michaelis–M...

Descripción completa

Detalles Bibliográficos
Autores principales:	Huang, Wei-Chun, Huang, Li-Juan, Hsu, Liang-Sheng, Huang, Shih-Ting, Lo, Wen-Ting, Wang, Tzu-Fan, Sun, Wei-Ting, Wei, Win-Yin, Lee, Ying-Shuan, Chuang, Shih-Hsien, Lee, Chao-Pin, Chou, Ho-Hsuan, Chen, Shu-Hui
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551328/ https://www.ncbi.nlm.nih.gov/pubmed/34707190 http://dx.doi.org/10.1038/s41598-021-00743-3

Ejemplares similares

A chiral phosphazane reagent strategy for the determination of enantiomeric excess of amines
por: Peel, Andrew J., et al.
Publicado: (2022)

Fetuin-A as a predicator of sarcopenic left ventricular dysfunction
por: Chang, Wei-Ting, et al.
Publicado: (2015)

Integration of High-Resolution Laser Displacement Sensors and 3D Printing for Structural Health Monitoring
por: Chang, Shu-Wei, et al.
Publicado: (2017)

Phenothiazinimides: Atom-Efficient Electrophilic Amination Reagents
por: Jin, Rongwei, et al.
Publicado: (2018)

Tumor-Infiltrating Leukocyte Composition and Prognostic Power in Hepatitis B- and Hepatitis C-Related Hepatocellular Carcinomas
por: Hsiao, Yi-Wen, et al.
Publicado: (2019)

Measurement of Cancer-Related Fatigue Based on Heart Rate Variability: Observational Study
por: Shih, Chi-Huang, et al.
Publicado: (2021)

Sulfinamide Synthesis Using Organometallic Reagents, DABSO, and Amines
por: Lo, Pui Kin Tony, et al.
Publicado: (2020)

Nitrenium ions as new versatile reagents for electrophilic amination
por: Avigdori, Idan, et al.
Publicado: (2023)

Aldosterone Excess Induced Mitochondria Decrease and Dysfunction via Mineralocorticoid Receptor and Oxidative Stress In Vitro and In Vivo
por: Tsai, Cheng-Hsuan, et al.
Publicado: (2021)

Predicative arithmetic (MN-32)
por: Nelson, Edward
Publicado: (2014)

Toward predicate approaches to modality
por: Stern, Johannes
Publicado: (2016)

Patterns and Trends in Semantic Predications
por: Chen, Chaomei, et al.
Publicado: (2017)

Argument-predicate distance as a filter for enhancing precision in extracting predications on the genetic etiology of disease
por: Masseroli, Marco, et al.
Publicado: (2006)

Predication of the Effector Proteins Secreted by Fusarium sacchari Using Genomic Analysis and Heterogenous Expression
por: Huang, Zhen, et al.
Publicado: (2022)

Registered nurses are at increased risk of hospitalization for infectious diseases and perinatal complications: A population-based observational study
por: Liao, Kuei-Lin, et al.
Publicado: (2019)

Aminals as powerful XAT-reagents: activation of fluorinated alkyl chlorides
por: Kostromitin, Vladislav S., et al.
Publicado: (2023)

Metasurface-empowered snapshot hyperspectral imaging with convex/deep (CODE) small-data learning theory
por: Lin, Chia-Hsiang, et al.
Publicado: (2023)

Clinical significance of circulating tumor cells in predicating the outcomes of patients with colorectal cancer
por: Chen, Kehe, et al.
Publicado: (2022)

High MET gene copy number predicted poor prognosis in primary intestinal diffuse large B-cell lymphoma
por: Huang, Wan-Ting, et al.
Publicado: (2013)

A Patient Friendly Corifollitropin Alfa Protocol without Routine Pituitary Suppression in Normal Responders
por: Wang, Huai-Ling, et al.
Publicado: (2016)

Predicate Oriented Pattern Analysis for Biomedical Knowledge Discovery
por: Shen, Feichen, et al.
Publicado: (2016)

Chest Ultrasound in Predication of Weaning Failure
por: Soliman, Soliman Belal, et al.
Publicado: (2019)

Bifunctional Reagents for Formylglycine Conjugation: Pitfalls and Breakthroughs
por: Janson, Nils, et al.
Publicado: (2020)

Meningioma Related Epilepsy- Pathophysiology, Pre/postoperative Seizures Predicators and Treatment
por: Elbadry Ahmed, Rasha, et al.
Publicado: (2022)

Reversibility of albuminuria and continuous positive airway pressure compliance in patients of obstructive sleep apnea syndrome
por: Chen, Ning-Hung, et al.
Publicado: (2016)

The Effectiveness of Isoplumbagin and Plumbagin in Regulating Amplitude, Gating Kinetics, and Voltage-Dependent Hysteresis of erg-mediated K(+) Currents
por: Chen, Linyi, et al.
Publicado: (2022)

Tertiary amine synthesis via reductive coupling of amides with Grignard reagents
por: Xie, Lan-Gui, et al.
Publicado: (2017)

Cobalt-Catalyzed (Hetero)arylation of Saturated Cyclic Amines with Grignard Reagents
por: Barré, Baptiste, et al.
Publicado: (2018)

Skeletal Editing—Nitrogen Deletion of Secondary Amines by Anomeric Amide Reagents
por: Zippel, Christoph, et al.
Publicado: (2021)

Amine–Boranes as Transfer Hydrogenation and Hydrogenation Reagents: A Mechanistic Perspective
por: Lau, Samantha, et al.
Publicado: (2021)

The advent of electrophilic hydroxylamine-derived reagents for the direct preparation of unprotected amines
por: Gasser, Valentina C. M., et al.
Publicado: (2022)

Mapping Peptide–Protein Interactions by Amine-Reactive Cleavable Photoaffinity Reagents
por: Korovesis, Dimitris, et al.
Publicado: (2023)

Dose-dependent genotype effects of BDNF Val66Met polymorphism on default mode network in early stage Alzheimer's disease
por: Lin, Pin-Hsuan, et al.
Publicado: (2016)

Identification of the Hepatic Metabolites of Flumazenil and their Kinetic Application in Neuroimaging
por: Chen, Wei-Hsi, et al.
Publicado: (2023)

Comparisons of Outcomes between Patients with Direct and Indirect Acute Respiratory Distress Syndrome Receiving Extracorporeal Membrane Oxygenation
por: Chiu, Li-Chung, et al.
Publicado: (2021)

A Sulfhydryl-Reactive Ruthenium (II) Complex and Its Conjugation to Protein G as a Universal Reagent for Fluorescent Immunoassays
por: Lin, Jing-Tang, et al.
Publicado: (2012)

Prognostic Value and Predication Model of Microvascular Invasion in Patients with Intrahepatic Cholangiocarcinoma
por: Tang, Zheng, et al.
Publicado: (2019)

Design and prototype of a mechanism for active on-line emerging/notifiable infectious diseases control, tracking and surveillance, based on a national healthcare card system
por: Huang, Jyh-Win, et al.
Publicado: (2007)

The performance of HCV GT plus RUO reagent in determining Hepatitis C virus genotypes in Taiwan
por: Huang, Ying-Chou, et al.
Publicado: (2021)

Main-chain engineering of polymer photocatalysts with hydrophilic non-conjugated segments for visible-light-driven hydrogen evolution
por: Chang, Chih-Li, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_d7829qvo18q5if32of6qrjgbqu