CRISPR deactivation in mammalian cells using photocleavable guide RNAs

The ability to deactivate CRISPR-Cas systems on demand would improve the safety and applicability of genome editing. Here, we detail a protocol using photocleavable guide RNAs (pcRNAs) to deactivate CRISPR-Cas9 inside cells. We verify that deactivation is both rapid and complete by checking for inse...

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Detalles Bibliográficos
Autores principales: Zou, Roger S., Liu, Yang, Ha, Taekjip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551926/
https://www.ncbi.nlm.nih.gov/pubmed/34746867
http://dx.doi.org/10.1016/j.xpro.2021.100909

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551926/
https://www.ncbi.nlm.nih.gov/pubmed/34746867
http://dx.doi.org/10.1016/j.xpro.2021.100909

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