Cargando…

Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells

High-resolution and rapid imaging of host cell ultrastructure can generate insights toward viral disease mechanism, for example for a severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. Here, we employ full-rotation soft X-ray tomography (SXT) to examine organelle remodeling indu...

Descripción completa

Detalles Bibliográficos
Autores principales:	Loconte, Valentina, Chen, Jian-Hua, Cortese, Mirko, Ekman, Axel, Le Gros, Mark A., Larabell, Carolyn, Bartenschlager, Ralf, Weinhardt, Venera
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8552653/ https://www.ncbi.nlm.nih.gov/pubmed/34729550 http://dx.doi.org/10.1016/j.crmeth.2021.100117

Ejemplares similares

Whole-cell quantitative imaging of structural changes induced by SARS-CoV-2 using soft X-ray tomography
por: Chen, Jian-Hua, et al.
Publicado: (2022)

A protocol for full-rotation soft X-ray tomography of single cells
por: Chen, Jian-Hua, et al.
Publicado: (2022)

Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells
por: Chen, Jian-Hua, et al.
Publicado: (2022)

Soft X‐ray tomograms provide a structural basis for whole‐cell modeling
por: Loconte, Valentina, et al.
Publicado: (2022)

Switchable resolution in soft x-ray tomography of single cells
por: Weinhardt, Venera, et al.
Publicado: (2020)

Visualizing subcellular rearrangements in intact β cells using soft x-ray tomography
por: White, Kate L., et al.
Publicado: (2020)

Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection
por: Aho, Vesa, et al.
Publicado: (2019)

The use of soft X-ray tomography to explore mitochondrial structure and function
por: Loconte, Valentina, et al.
Publicado: (2021)

Quantitative morphometric analysis of adult teleost fish by X-ray computed tomography
por: Weinhardt, Venera, et al.
Publicado: (2018)

Cryo-soft X-ray tomography: using soft X-rays to explore the ultrastructure of whole cells
por: Harkiolaki, Maria, et al.
Publicado: (2018)

An intensity-based post-processing tool for 3D instance segmentation of organelles in soft X-ray tomograms
por: Li, Angdi, et al.
Publicado: (2022)

Three-dimensional imaging of mitochondrial cristae complexity using cryo-soft X-ray tomography
por: Polo, Carla C., et al.
Publicado: (2020)

3D Ultrastructural Organization of Whole Chlamydomonas reinhardtii Cells Studied by Nanoscale Soft X-Ray Tomography
por: Hummel, Eric, et al.
Publicado: (2012)

Imaging of Virus-Infected Cells with Soft X-ray Tomography
por: Garriga, Damià, et al.
Publicado: (2021)

Cryo-soft X-ray tomography as a quantitative three-dimensional tool to model nanoparticle:cell interaction
por: Chiappi, Michele, et al.
Publicado: (2016)

GPU-accelerated ray-casting for 3D fiber orientation analysis
por: Shkarin, Roman, et al.
Publicado: (2020)

Resonant Inelastic Soft X-ray Scattering and X-ray Emission Spectroscopy of Solid Proline and Proline Solutions
por: Meyer, Frank, et al.
Publicado: (2022)

Rewiring cellular networks by members of the Flaviviridae family
por: Neufeldt, Christopher J., et al.
Publicado: (2018)

Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties
por: Boeynaems, Steven, et al.
Publicado: (2019)

Dengue Virus Non-structural Protein 1 Modulates Infectious Particle Production via Interaction with the Structural Proteins
por: Scaturro, Pietro, et al.
Publicado: (2015)

The cellular landscape by cryo soft X-ray tomography
por: Groen, J., et al.
Publicado: (2019)

SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography
por: Klein, Steffen, et al.
Publicado: (2020)

Correlative VIS-fluorescence and soft X-ray cryo-microscopy/tomography of adherent cells
por: Hagen, Christoph, et al.
Publicado: (2012)

Cryo-soft X-ray tomography: a journey into the world of the native-state cell
por: Carzaniga, Raffaella, et al.
Publicado: (2013)

Strong intracellular signal inactivation produces sharper and more robust signaling from cell membrane to nucleus
por: Ma, Jingwei, et al.
Publicado: (2020)

Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles
por: Goellner, Sarah, et al.
Publicado: (2020)

Quantitative X-ray Tomography of the Mouse Cochlea
por: Rau, Christoph, et al.
Publicado: (2012)

Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling
por: Remesh, Soumya G., et al.
Publicado: (2020)

Quantitative disentanglement of nanocrystalline phases in cement pastes by synchrotron ptychographic X-ray tomography
por: Cuesta, Ana, et al.
Publicado: (2019)

A Versatile Reporter System To Monitor Virus-Infected Cells and Its Application to Dengue Virus and SARS-CoV-2
por: Pahmeier, Felix, et al.
Publicado: (2021)

Machine learning assists in increasing the time resolution of X-ray computed tomography applied to mineral precipitation in porous media
por: Lee, Dongwon, et al.
Publicado: (2023)

Microscopic Visualization of Cell-Cell Adhesion Complexes at Micro and Nanoscale
por: Vanslembrouck, Bieke, et al.
Publicado: (2022)

Soft X-ray tomography during ELMs and impurity injection in JET
por: Ingesson, L C, et al.
Publicado: (1998)

XTEND: Extending the depth of field in cryo soft X-ray tomography
por: Otón, Joaquín, et al.
Publicado: (2017)

Nanoimaging granule dynamics and subcellular structures in activated mast cells using soft X-ray tomography
por: Chen, Huan-Yuan, et al.
Publicado: (2016)

On-Line Fluorescence Microscopy for Identification and Imaging of Apoptotic Cell with Synchrotron-Based Soft X-ray Tomography
por: Zhang, Chao, et al.
Publicado: (2023)

A Reverse Genetics System for Zika Virus Based on a Simple Molecular Cloning Strategy
por: Münster, Maximilian, et al.
Publicado: (2018)

Corrigendum: Nanoimaging granule dynamics and subcellular structures in activated mast cells using soft X-ray tomography
por: Chen, Huan-Yuan, et al.
Publicado: (2017)

Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development
por: Zilova, Lucie, et al.
Publicado: (2021)

Critical Role of Type III Interferon in Controlling SARS-CoV-2 Infection in Human Intestinal Epithelial Cells
por: Stanifer, Megan L., et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_5775mrhguugrfu6tt1i851b8l5