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Metabolite Profiling of Meridianin C In Vivo of Rat by UHPLC/Q-TOF MS
Meridianin C (MC), as a marine alkaloid, is a potent protein kinase inhibitor which exhibits good anticancer activity. However, the in vivo metabolism of MC has not been described to date. In this study, an ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHP...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553504/ https://www.ncbi.nlm.nih.gov/pubmed/34721922 http://dx.doi.org/10.1155/2021/1382421 |
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author | Zhang, Guozhe Xiao, Linxia Qi, Liang |
author_facet | Zhang, Guozhe Xiao, Linxia Qi, Liang |
author_sort | Zhang, Guozhe |
collection | PubMed |
description | Meridianin C (MC), as a marine alkaloid, is a potent protein kinase inhibitor which exhibits good anticancer activity. However, the in vivo metabolism of MC has not been described to date. In this study, an ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF MS) method is employed to investigate the in vivo metabolites of MC in rats. Plasma, bile, urine, and feces are collected after a single oral dose of MC. Protein precipitation, solid phase extraction (SPE), and ultrasonic extraction methods are used to prepare samples. Based on the mass spectral fragmentation patterns, elution order, and retrieving literatures, a total of 13 metabolites of MC were detected and tentatively identified, utilizing MetaboLynx software. The metabolic pathways of MC in rats include N- or O-glucuronidation, O-sulfation, N-hydroxylation, dihydroxylation, and trihydroxylation. The relative content of the metabolites in each kinds of biological samples is also evaluated. This study will help to understand the in vivo properties of MC for the future usage. |
format | Online Article Text |
id | pubmed-8553504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-85535042021-10-29 Metabolite Profiling of Meridianin C In Vivo of Rat by UHPLC/Q-TOF MS Zhang, Guozhe Xiao, Linxia Qi, Liang J Anal Methods Chem Research Article Meridianin C (MC), as a marine alkaloid, is a potent protein kinase inhibitor which exhibits good anticancer activity. However, the in vivo metabolism of MC has not been described to date. In this study, an ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF MS) method is employed to investigate the in vivo metabolites of MC in rats. Plasma, bile, urine, and feces are collected after a single oral dose of MC. Protein precipitation, solid phase extraction (SPE), and ultrasonic extraction methods are used to prepare samples. Based on the mass spectral fragmentation patterns, elution order, and retrieving literatures, a total of 13 metabolites of MC were detected and tentatively identified, utilizing MetaboLynx software. The metabolic pathways of MC in rats include N- or O-glucuronidation, O-sulfation, N-hydroxylation, dihydroxylation, and trihydroxylation. The relative content of the metabolites in each kinds of biological samples is also evaluated. This study will help to understand the in vivo properties of MC for the future usage. Hindawi 2021-10-21 /pmc/articles/PMC8553504/ /pubmed/34721922 http://dx.doi.org/10.1155/2021/1382421 Text en Copyright © 2021 Guozhe Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhang, Guozhe Xiao, Linxia Qi, Liang Metabolite Profiling of Meridianin C In Vivo of Rat by UHPLC/Q-TOF MS |
title | Metabolite Profiling of Meridianin C In Vivo of Rat by UHPLC/Q-TOF MS |
title_full | Metabolite Profiling of Meridianin C In Vivo of Rat by UHPLC/Q-TOF MS |
title_fullStr | Metabolite Profiling of Meridianin C In Vivo of Rat by UHPLC/Q-TOF MS |
title_full_unstemmed | Metabolite Profiling of Meridianin C In Vivo of Rat by UHPLC/Q-TOF MS |
title_short | Metabolite Profiling of Meridianin C In Vivo of Rat by UHPLC/Q-TOF MS |
title_sort | metabolite profiling of meridianin c in vivo of rat by uhplc/q-tof ms |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553504/ https://www.ncbi.nlm.nih.gov/pubmed/34721922 http://dx.doi.org/10.1155/2021/1382421 |
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