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The rates of stem cell division determine the cell cycle lengths of its lineage

Adult stem cells and their transit-amplifying progeny alter their proliferation rates to maintain tissue homeostasis. To test how the division rates of stem cells and transit-amplifying progeny affect tissue growth and differentiation, we developed a computation strategy that estimates the average c...

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Autores principales: Gadre, Purna, Nitsure, Nitin, Mazumdar, Debasmita, Gupta, Samir, Ray, Krishanu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555441/
https://www.ncbi.nlm.nih.gov/pubmed/34746698
http://dx.doi.org/10.1016/j.isci.2021.103232
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author Gadre, Purna
Nitsure, Nitin
Mazumdar, Debasmita
Gupta, Samir
Ray, Krishanu
author_facet Gadre, Purna
Nitsure, Nitin
Mazumdar, Debasmita
Gupta, Samir
Ray, Krishanu
author_sort Gadre, Purna
collection PubMed
description Adult stem cells and their transit-amplifying progeny alter their proliferation rates to maintain tissue homeostasis. To test how the division rates of stem cells and transit-amplifying progeny affect tissue growth and differentiation, we developed a computation strategy that estimates the average cell-cycle lengths (lifespans) of germline stem cells and their progeny from fixed-tissue demography in the Drosophila testis. Analysis of the wild-type data using this method indicated that during the germline transit-amplification, the cellular lifespans extend by nearly 1.3-fold after the first division and shrink by about 2-folds after the second division. Cell-autonomous perturbations of the stem cell lifespan accordingly altered the lifespans of successive transit-amplifying stages. Remarkably, almost 2-fold alterations in the lifespans of stem cells and their immediate daughters did not affect the subsequent differentiation. The results indicate that the early germline division rates can adjust the following division rates and the onset of differentiation.
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spelling pubmed-85554412021-11-05 The rates of stem cell division determine the cell cycle lengths of its lineage Gadre, Purna Nitsure, Nitin Mazumdar, Debasmita Gupta, Samir Ray, Krishanu iScience Article Adult stem cells and their transit-amplifying progeny alter their proliferation rates to maintain tissue homeostasis. To test how the division rates of stem cells and transit-amplifying progeny affect tissue growth and differentiation, we developed a computation strategy that estimates the average cell-cycle lengths (lifespans) of germline stem cells and their progeny from fixed-tissue demography in the Drosophila testis. Analysis of the wild-type data using this method indicated that during the germline transit-amplification, the cellular lifespans extend by nearly 1.3-fold after the first division and shrink by about 2-folds after the second division. Cell-autonomous perturbations of the stem cell lifespan accordingly altered the lifespans of successive transit-amplifying stages. Remarkably, almost 2-fold alterations in the lifespans of stem cells and their immediate daughters did not affect the subsequent differentiation. The results indicate that the early germline division rates can adjust the following division rates and the onset of differentiation. Elsevier 2021-10-06 /pmc/articles/PMC8555441/ /pubmed/34746698 http://dx.doi.org/10.1016/j.isci.2021.103232 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gadre, Purna
Nitsure, Nitin
Mazumdar, Debasmita
Gupta, Samir
Ray, Krishanu
The rates of stem cell division determine the cell cycle lengths of its lineage
title The rates of stem cell division determine the cell cycle lengths of its lineage
title_full The rates of stem cell division determine the cell cycle lengths of its lineage
title_fullStr The rates of stem cell division determine the cell cycle lengths of its lineage
title_full_unstemmed The rates of stem cell division determine the cell cycle lengths of its lineage
title_short The rates of stem cell division determine the cell cycle lengths of its lineage
title_sort rates of stem cell division determine the cell cycle lengths of its lineage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555441/
https://www.ncbi.nlm.nih.gov/pubmed/34746698
http://dx.doi.org/10.1016/j.isci.2021.103232
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