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The rates of stem cell division determine the cell cycle lengths of its lineage
Adult stem cells and their transit-amplifying progeny alter their proliferation rates to maintain tissue homeostasis. To test how the division rates of stem cells and transit-amplifying progeny affect tissue growth and differentiation, we developed a computation strategy that estimates the average c...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555441/ https://www.ncbi.nlm.nih.gov/pubmed/34746698 http://dx.doi.org/10.1016/j.isci.2021.103232 |
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author | Gadre, Purna Nitsure, Nitin Mazumdar, Debasmita Gupta, Samir Ray, Krishanu |
author_facet | Gadre, Purna Nitsure, Nitin Mazumdar, Debasmita Gupta, Samir Ray, Krishanu |
author_sort | Gadre, Purna |
collection | PubMed |
description | Adult stem cells and their transit-amplifying progeny alter their proliferation rates to maintain tissue homeostasis. To test how the division rates of stem cells and transit-amplifying progeny affect tissue growth and differentiation, we developed a computation strategy that estimates the average cell-cycle lengths (lifespans) of germline stem cells and their progeny from fixed-tissue demography in the Drosophila testis. Analysis of the wild-type data using this method indicated that during the germline transit-amplification, the cellular lifespans extend by nearly 1.3-fold after the first division and shrink by about 2-folds after the second division. Cell-autonomous perturbations of the stem cell lifespan accordingly altered the lifespans of successive transit-amplifying stages. Remarkably, almost 2-fold alterations in the lifespans of stem cells and their immediate daughters did not affect the subsequent differentiation. The results indicate that the early germline division rates can adjust the following division rates and the onset of differentiation. |
format | Online Article Text |
id | pubmed-8555441 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-85554412021-11-05 The rates of stem cell division determine the cell cycle lengths of its lineage Gadre, Purna Nitsure, Nitin Mazumdar, Debasmita Gupta, Samir Ray, Krishanu iScience Article Adult stem cells and their transit-amplifying progeny alter their proliferation rates to maintain tissue homeostasis. To test how the division rates of stem cells and transit-amplifying progeny affect tissue growth and differentiation, we developed a computation strategy that estimates the average cell-cycle lengths (lifespans) of germline stem cells and their progeny from fixed-tissue demography in the Drosophila testis. Analysis of the wild-type data using this method indicated that during the germline transit-amplification, the cellular lifespans extend by nearly 1.3-fold after the first division and shrink by about 2-folds after the second division. Cell-autonomous perturbations of the stem cell lifespan accordingly altered the lifespans of successive transit-amplifying stages. Remarkably, almost 2-fold alterations in the lifespans of stem cells and their immediate daughters did not affect the subsequent differentiation. The results indicate that the early germline division rates can adjust the following division rates and the onset of differentiation. Elsevier 2021-10-06 /pmc/articles/PMC8555441/ /pubmed/34746698 http://dx.doi.org/10.1016/j.isci.2021.103232 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gadre, Purna Nitsure, Nitin Mazumdar, Debasmita Gupta, Samir Ray, Krishanu The rates of stem cell division determine the cell cycle lengths of its lineage |
title | The rates of stem cell division determine the cell cycle lengths of its lineage |
title_full | The rates of stem cell division determine the cell cycle lengths of its lineage |
title_fullStr | The rates of stem cell division determine the cell cycle lengths of its lineage |
title_full_unstemmed | The rates of stem cell division determine the cell cycle lengths of its lineage |
title_short | The rates of stem cell division determine the cell cycle lengths of its lineage |
title_sort | rates of stem cell division determine the cell cycle lengths of its lineage |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555441/ https://www.ncbi.nlm.nih.gov/pubmed/34746698 http://dx.doi.org/10.1016/j.isci.2021.103232 |
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