Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants

BACKGROUND: Multiplex CRISPR-Cas9-based genome editing is an efficient method for targeted disruption of gene function in plants. Use of CRISPR-Cas9 has increased rapidly in recent years and is becoming a routine method for generating single and higher order Arabidopsis thaliana mutants. Low entry,...

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Detalles Bibliográficos
Autores principales: Ursache, Robertas, Fujita, Satoshi, Dénervaud Tendon, Valérie, Geldner, Niko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556964/
https://www.ncbi.nlm.nih.gov/pubmed/34717688
http://dx.doi.org/10.1186/s13007-021-00811-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556964/
https://www.ncbi.nlm.nih.gov/pubmed/34717688
http://dx.doi.org/10.1186/s13007-021-00811-9

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