Targeted regulation of transcription in primary cells using CRISPRa and CRISPRi

Targeted transcriptional activation or interference can be induced with the CRISPR-Cas9 system (CRISPRa/CRISPRi) using nuclease-deactivated Cas9 fused to transcriptional effector molecules. These technologies have been used in cancer cell lines, particularly for genome-wide functional genetic screen...

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Detalles Bibliográficos
Autores principales: Jensen, Trine I., Mikkelsen, Nanna S., Gao, Zongliang, Foßelteder, Johannes, Pabst, Gabriel, Axelgaard, Esben, Laustsen, Anders, König, Saskia, Reinisch, Andreas, Bak, Rasmus O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2021
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8559706/
https://www.ncbi.nlm.nih.gov/pubmed/34407984
http://dx.doi.org/10.1101/gr.275607.121

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8559706/
https://www.ncbi.nlm.nih.gov/pubmed/34407984
http://dx.doi.org/10.1101/gr.275607.121

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