Novel Disk Diffusion Assay on Magnesium Oxalate Agar To Evaluate the Susceptibility of Yersinia pestis to Type III Secretion System Inhibitors

Current methods for screening small molecules that inhibit the plasmid pCD1-encoded Yersinia pestis type III secretion system (T3SS) include lengthy growth curves followed by multistep luminescence assays or Western blot assays to detect secretion, or lack thereof, of effector proteins. The goal of...

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Detalles Bibliográficos
Autores principales: Prashar, Sukriti, Portales Guemes, Miguel, Shivbaran, Poorandai, Jimenez Alvarez, Eugenia, Soha, Christopher, Nacer, Samir, McDonough, Michael, Plano, Gregory V., Torruellas Garcia, Julie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Methods and Protocols
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8562340/
https://www.ncbi.nlm.nih.gov/pubmed/34132578
http://dx.doi.org/10.1128/spectrum.00005-21

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8562340/
https://www.ncbi.nlm.nih.gov/pubmed/34132578
http://dx.doi.org/10.1128/spectrum.00005-21

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