High-resolution single-particle cryo-EM of samples vitrified in boiling nitro­gen

Based on work by Dubochet and others in the 1980s and 1990s, samples for single-particle cryo-electron microscopy (cryo-EM) have been vitrified using ethane, propane or ethane/propane mixtures. These liquid cryogens have a large difference between their melting and boiling temperatures and so can ab...

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Detalles Bibliográficos
Autores principales: Engstrom, Tyler, Clinger, Jonathan A., Spoth, Katherine A., Clarke, Oliver B., Closs, David S., Jayne, Richard, Apker, Benjamin A., Thorne, Robert E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2021
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8562666/
https://www.ncbi.nlm.nih.gov/pubmed/34804541
http://dx.doi.org/10.1107/S2052252521008095

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8562666/
https://www.ncbi.nlm.nih.gov/pubmed/34804541
http://dx.doi.org/10.1107/S2052252521008095

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