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The effect of light on follicular development in laying hens
OBJECTIVE: The oxidative stress status and changes of chicken ovary tissue after shading were studied, to determine the mechanism of the effect of shading on follicular development. METHODS: Twenty healthy laying hens (40 weeks old) with uniform body weight and the same laying rate were randomly div...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Animal Bioscience
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563248/ https://www.ncbi.nlm.nih.gov/pubmed/33848416 http://dx.doi.org/10.5713/ab.20.0791 |
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author | Cheng, Shi Bin Li, Xian Qiang Wang, Jia Xiang Wu, Yan Li, Peng Pi, Jin Song |
author_facet | Cheng, Shi Bin Li, Xian Qiang Wang, Jia Xiang Wu, Yan Li, Peng Pi, Jin Song |
author_sort | Cheng, Shi Bin |
collection | PubMed |
description | OBJECTIVE: The oxidative stress status and changes of chicken ovary tissue after shading were studied, to determine the mechanism of the effect of shading on follicular development. METHODS: Twenty healthy laying hens (40 weeks old) with uniform body weight and the same laying rate were randomly divided into two groups (the shading group and normal light group). In the shading group, the cage was covered to reduce the light intensity inside the cage to 0 without affecting ventilation or food intake. The normal lighting group received no additional treatment. After 7 days of shading, oxidative stress related indicators and gene expression were detected. RESULTS: Analysis of paraffin and ultrathin sections showed that apoptosis of ovarian granulosa cells (GCs) increased significantly after light shading. Enzyme linked immunosorbent assay results revealed that the levels of total antioxidant capacity, malondialdehyde, superoxide dismutase (SOD), glutathione, catalase (CAT), and other substances in the sera, livers, ovaries, and follicular GCs of laying hens increased significantly after shading for 7 days; and reactive oxygen species (ROS) levels in the livers of laying hens also increased significantly. ROS in the serum, ovarian and GCs also increased. After shading for 7 days, the levels of 8-hydroxy-2 deoxyguanosine in the sera and ovarian tissues of laying hens increased significantly. Cell counting kit-8 detection showed that the proliferation activity of GCs in layer follicles decreased after shading for 7 days; the expression level of the anti-apoptotic gene B-cell lymphoma-2 in ovarian tissue and follicular GCs was significantly reduced, and the expression levels of pro-apoptotic caspase 3 (casp3), and SOD, glutathione peroxidase 2 (GPX2), and CAT were all significantly increased. CONCLUSION: Oxidative stress induced by shading light has a serious inhibitory effect on follicular development during reproduction in laying hens. |
format | Online Article Text |
id | pubmed-8563248 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Animal Bioscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-85632482021-11-17 The effect of light on follicular development in laying hens Cheng, Shi Bin Li, Xian Qiang Wang, Jia Xiang Wu, Yan Li, Peng Pi, Jin Song Anim Biosci Article OBJECTIVE: The oxidative stress status and changes of chicken ovary tissue after shading were studied, to determine the mechanism of the effect of shading on follicular development. METHODS: Twenty healthy laying hens (40 weeks old) with uniform body weight and the same laying rate were randomly divided into two groups (the shading group and normal light group). In the shading group, the cage was covered to reduce the light intensity inside the cage to 0 without affecting ventilation or food intake. The normal lighting group received no additional treatment. After 7 days of shading, oxidative stress related indicators and gene expression were detected. RESULTS: Analysis of paraffin and ultrathin sections showed that apoptosis of ovarian granulosa cells (GCs) increased significantly after light shading. Enzyme linked immunosorbent assay results revealed that the levels of total antioxidant capacity, malondialdehyde, superoxide dismutase (SOD), glutathione, catalase (CAT), and other substances in the sera, livers, ovaries, and follicular GCs of laying hens increased significantly after shading for 7 days; and reactive oxygen species (ROS) levels in the livers of laying hens also increased significantly. ROS in the serum, ovarian and GCs also increased. After shading for 7 days, the levels of 8-hydroxy-2 deoxyguanosine in the sera and ovarian tissues of laying hens increased significantly. Cell counting kit-8 detection showed that the proliferation activity of GCs in layer follicles decreased after shading for 7 days; the expression level of the anti-apoptotic gene B-cell lymphoma-2 in ovarian tissue and follicular GCs was significantly reduced, and the expression levels of pro-apoptotic caspase 3 (casp3), and SOD, glutathione peroxidase 2 (GPX2), and CAT were all significantly increased. CONCLUSION: Oxidative stress induced by shading light has a serious inhibitory effect on follicular development during reproduction in laying hens. Animal Bioscience 2021-11 2021-04-13 /pmc/articles/PMC8563248/ /pubmed/33848416 http://dx.doi.org/10.5713/ab.20.0791 Text en Copyright © 2021 by Animal Bioscience https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Cheng, Shi Bin Li, Xian Qiang Wang, Jia Xiang Wu, Yan Li, Peng Pi, Jin Song The effect of light on follicular development in laying hens |
title | The effect of light on follicular development in laying hens |
title_full | The effect of light on follicular development in laying hens |
title_fullStr | The effect of light on follicular development in laying hens |
title_full_unstemmed | The effect of light on follicular development in laying hens |
title_short | The effect of light on follicular development in laying hens |
title_sort | effect of light on follicular development in laying hens |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563248/ https://www.ncbi.nlm.nih.gov/pubmed/33848416 http://dx.doi.org/10.5713/ab.20.0791 |
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