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Peptide barcoding for one-pot evaluation of sequence–function relationships of nanobodies

Optimisation of protein binders relies on laborious screening processes. Investigation of sequence–function relationships of protein binders is particularly slow, since mutants are purified and evaluated individually. Here we developed peptide barcoding, a high-throughput approach for accurate inves...

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Detalles Bibliográficos
Autores principales: Matsuzaki, Yusei, Aoki, Wataru, Miyazaki, Takumi, Aburaya, Shunsuke, Ohtani, Yuta, Kajiwara, Kaho, Koike, Naoki, Minakuchi, Hiroyoshi, Miura, Natsuko, Kadonosono, Tetsuya, Ueda, Mitsuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563947/
https://www.ncbi.nlm.nih.gov/pubmed/34728738
http://dx.doi.org/10.1038/s41598-021-01019-6