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Peptide barcoding for one-pot evaluation of sequence–function relationships of nanobodies
Optimisation of protein binders relies on laborious screening processes. Investigation of sequence–function relationships of protein binders is particularly slow, since mutants are purified and evaluated individually. Here we developed peptide barcoding, a high-throughput approach for accurate inves...
Autores principales: | Matsuzaki, Yusei, Aoki, Wataru, Miyazaki, Takumi, Aburaya, Shunsuke, Ohtani, Yuta, Kajiwara, Kaho, Koike, Naoki, Minakuchi, Hiroyoshi, Miura, Natsuko, Kadonosono, Tetsuya, Ueda, Mitsuyoshi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563947/ https://www.ncbi.nlm.nih.gov/pubmed/34728738 http://dx.doi.org/10.1038/s41598-021-01019-6 |
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