Cargando…

Absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer

Measuring the aberrations of optical systems is an essential step in the fabrication of high precision optical components. Such a characterization is usually based on comparing the device under investigation with a calibrated reference object. However, when working at the cutting-edge of technology,...

Descripción completa

Detalles Bibliográficos
Autores principales: Eismann, Jörg S., Neugebauer, Martin, Mantel, Klaus, Banzer, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563975/
https://www.ncbi.nlm.nih.gov/pubmed/34728608
http://dx.doi.org/10.1038/s41377-021-00663-x
_version_ 1784593516284870656
author Eismann, Jörg S.
Neugebauer, Martin
Mantel, Klaus
Banzer, Peter
author_facet Eismann, Jörg S.
Neugebauer, Martin
Mantel, Klaus
Banzer, Peter
author_sort Eismann, Jörg S.
collection PubMed
description Measuring the aberrations of optical systems is an essential step in the fabrication of high precision optical components. Such a characterization is usually based on comparing the device under investigation with a calibrated reference object. However, when working at the cutting-edge of technology, it is increasingly difficult to provide an even better or well-known reference device. In this manuscript we present a method for the characterization of high numerical aperture microscope objectives, functioning without the need of calibrated reference optics. The technique constitutes a nanoparticle, acting as a dipole-like scatterer, that is placed in the focal volume of the microscope objective. The light that is scattered by the particle can be measured individually and serves as the reference wave in our system. Utilizing the well-characterized scattered light as nearly perfect reference wave is the main idea behind this manuscript.
format Online
Article
Text
id pubmed-8563975
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-85639752021-11-16 Absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer Eismann, Jörg S. Neugebauer, Martin Mantel, Klaus Banzer, Peter Light Sci Appl Article Measuring the aberrations of optical systems is an essential step in the fabrication of high precision optical components. Such a characterization is usually based on comparing the device under investigation with a calibrated reference object. However, when working at the cutting-edge of technology, it is increasingly difficult to provide an even better or well-known reference device. In this manuscript we present a method for the characterization of high numerical aperture microscope objectives, functioning without the need of calibrated reference optics. The technique constitutes a nanoparticle, acting as a dipole-like scatterer, that is placed in the focal volume of the microscope objective. The light that is scattered by the particle can be measured individually and serves as the reference wave in our system. Utilizing the well-characterized scattered light as nearly perfect reference wave is the main idea behind this manuscript. Nature Publishing Group UK 2021-11-02 /pmc/articles/PMC8563975/ /pubmed/34728608 http://dx.doi.org/10.1038/s41377-021-00663-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Eismann, Jörg S.
Neugebauer, Martin
Mantel, Klaus
Banzer, Peter
Absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer
title Absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer
title_full Absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer
title_fullStr Absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer
title_full_unstemmed Absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer
title_short Absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer
title_sort absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563975/
https://www.ncbi.nlm.nih.gov/pubmed/34728608
http://dx.doi.org/10.1038/s41377-021-00663-x
work_keys_str_mv AT eismannjorgs absolutecharacterizationofhighnumericalaperturemicroscopeobjectivesutilizingadipolescatterer
AT neugebauermartin absolutecharacterizationofhighnumericalaperturemicroscopeobjectivesutilizingadipolescatterer
AT mantelklaus absolutecharacterizationofhighnumericalaperturemicroscopeobjectivesutilizingadipolescatterer
AT banzerpeter absolutecharacterizationofhighnumericalaperturemicroscopeobjectivesutilizingadipolescatterer