Cargando…
Absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer
Measuring the aberrations of optical systems is an essential step in the fabrication of high precision optical components. Such a characterization is usually based on comparing the device under investigation with a calibrated reference object. However, when working at the cutting-edge of technology,...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563975/ https://www.ncbi.nlm.nih.gov/pubmed/34728608 http://dx.doi.org/10.1038/s41377-021-00663-x |
_version_ | 1784593516284870656 |
---|---|
author | Eismann, Jörg S. Neugebauer, Martin Mantel, Klaus Banzer, Peter |
author_facet | Eismann, Jörg S. Neugebauer, Martin Mantel, Klaus Banzer, Peter |
author_sort | Eismann, Jörg S. |
collection | PubMed |
description | Measuring the aberrations of optical systems is an essential step in the fabrication of high precision optical components. Such a characterization is usually based on comparing the device under investigation with a calibrated reference object. However, when working at the cutting-edge of technology, it is increasingly difficult to provide an even better or well-known reference device. In this manuscript we present a method for the characterization of high numerical aperture microscope objectives, functioning without the need of calibrated reference optics. The technique constitutes a nanoparticle, acting as a dipole-like scatterer, that is placed in the focal volume of the microscope objective. The light that is scattered by the particle can be measured individually and serves as the reference wave in our system. Utilizing the well-characterized scattered light as nearly perfect reference wave is the main idea behind this manuscript. |
format | Online Article Text |
id | pubmed-8563975 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85639752021-11-16 Absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer Eismann, Jörg S. Neugebauer, Martin Mantel, Klaus Banzer, Peter Light Sci Appl Article Measuring the aberrations of optical systems is an essential step in the fabrication of high precision optical components. Such a characterization is usually based on comparing the device under investigation with a calibrated reference object. However, when working at the cutting-edge of technology, it is increasingly difficult to provide an even better or well-known reference device. In this manuscript we present a method for the characterization of high numerical aperture microscope objectives, functioning without the need of calibrated reference optics. The technique constitutes a nanoparticle, acting as a dipole-like scatterer, that is placed in the focal volume of the microscope objective. The light that is scattered by the particle can be measured individually and serves as the reference wave in our system. Utilizing the well-characterized scattered light as nearly perfect reference wave is the main idea behind this manuscript. Nature Publishing Group UK 2021-11-02 /pmc/articles/PMC8563975/ /pubmed/34728608 http://dx.doi.org/10.1038/s41377-021-00663-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Eismann, Jörg S. Neugebauer, Martin Mantel, Klaus Banzer, Peter Absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer |
title | Absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer |
title_full | Absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer |
title_fullStr | Absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer |
title_full_unstemmed | Absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer |
title_short | Absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer |
title_sort | absolute characterization of high numerical aperture microscope objectives utilizing a dipole scatterer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563975/ https://www.ncbi.nlm.nih.gov/pubmed/34728608 http://dx.doi.org/10.1038/s41377-021-00663-x |
work_keys_str_mv | AT eismannjorgs absolutecharacterizationofhighnumericalaperturemicroscopeobjectivesutilizingadipolescatterer AT neugebauermartin absolutecharacterizationofhighnumericalaperturemicroscopeobjectivesutilizingadipolescatterer AT mantelklaus absolutecharacterizationofhighnumericalaperturemicroscopeobjectivesutilizingadipolescatterer AT banzerpeter absolutecharacterizationofhighnumericalaperturemicroscopeobjectivesutilizingadipolescatterer |