Detection, sorting, and immortalization of dual expresser lymphocytes from human peripheral blood samples

This protocol describes how to identify Dual Expressers (DEs), a rare type of lymphocyte that co-expresses B-cell receptors and T-cell receptors, by flow cytometry using a cocktail of four antibodies. It also shows the subsequent gating strategy for detecting and sorting DEs and the generation of EB...

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Detalles Bibliográficos
Autores principales: Ahmed, Rizwan, Ananth, Kusuma, Omidian, Zahra, Majety, Neha, Zhang, Hao, Hamad, Abdel Rahim A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564191/
https://www.ncbi.nlm.nih.gov/pubmed/34755121
http://dx.doi.org/10.1016/j.xpro.2021.100925

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564191/
https://www.ncbi.nlm.nih.gov/pubmed/34755121
http://dx.doi.org/10.1016/j.xpro.2021.100925

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