Protocol for labeling and fixation of intact lysosomes with esterified amino acid analogs to assess lysosomal expansion in living eukaryotic cells

The lysosomal compartment is a key hub for cell metabolism, and morphological alterations have been described in several pathological conditions. Here, we describe the use of amino acid analogs modified by the presence of a methyl ester group that accumulates within lysosomes. This generates an intr...

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Detalles Bibliográficos
Autores principales: Scerra, Gianluca, Caporaso, Maria Gabriella, Renna, Maurizio, D’Agostino, Massimo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564676/
https://www.ncbi.nlm.nih.gov/pubmed/34755119
http://dx.doi.org/10.1016/j.xpro.2021.100916

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564676/
https://www.ncbi.nlm.nih.gov/pubmed/34755119
http://dx.doi.org/10.1016/j.xpro.2021.100916

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