Quantitative assessment of engineered Cas9 variants for target specificity enhancement by single-molecule reaction pathway analysis

There have been many engineered Cas9 variants that were developed to minimize unintended cleavage of off-target DNAs, but detailed mechanism for the way they regulate the target specificity through DNA:RNA heteroduplexation remains poorly understood. We used single-molecule FRET assay to follow the...

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Detalles Bibliográficos
Autores principales: Bak, So Young, Jung, Youngri, Park, Jinho, Sung, Keewon, Jang, Hyeon-Ki, Bae, Sangsu, Kim, Seong Keun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
RNA and RNA-protein complexes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565309/
https://www.ncbi.nlm.nih.gov/pubmed/34554247
http://dx.doi.org/10.1093/nar/gkab858

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565309/
https://www.ncbi.nlm.nih.gov/pubmed/34554247
http://dx.doi.org/10.1093/nar/gkab858

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