Detection of granzyme B in CD3-positive cells infiltrated in lesional skin of a dog with erythema multiforme associated with zonisamide

In human erythema multiforme (EM), cytotoxic T lymphocytes (CTLs) play an essential role in the pathogenesis. In canine EM, immunohistochemical staining with anti-CD8 antibody using frozen sections has shown the involvement of CTLs; however, CTL infiltration has never been quantitatively analyzed. W...

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Autores principales: TANAKA, Nana, KANEI, Toshitaka, IWATA, Munetaka, KAWABE, Mifumi, KAMISHINA, Hiroaki, MURAKAMI, Mami, SAKAI, Hiroki, MAEDA, Sadatoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2021
Materias:
Internal Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569875/
https://www.ncbi.nlm.nih.gov/pubmed/34433730
http://dx.doi.org/10.1292/jvms.21-0146
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author TANAKA, Nana
KANEI, Toshitaka
IWATA, Munetaka
KAWABE, Mifumi
KAMISHINA, Hiroaki
MURAKAMI, Mami
SAKAI, Hiroki
MAEDA, Sadatoshi
author_facet TANAKA, Nana
KANEI, Toshitaka
IWATA, Munetaka
KAWABE, Mifumi
KAMISHINA, Hiroaki
MURAKAMI, Mami
SAKAI, Hiroki
MAEDA, Sadatoshi
author_sort TANAKA, Nana
collection PubMed
description In human erythema multiforme (EM), cytotoxic T lymphocytes (CTLs) play an essential role in the pathogenesis. In canine EM, immunohistochemical staining with anti-CD8 antibody using frozen sections has shown the involvement of CTLs; however, CTL infiltration has never been quantitatively analyzed. We herein quantitatively analyzed CTL infiltration by immunohistochemical staining with granzyme B and CD3 antibodies using paraffin sections of a dog with EM associated with zonisamide. The present results indicated approximately 70% of cells at the border between the epidermis and dermis consisted of CTLs. Detection of granzyme B and CD3 using paraffin sections employed in this study can be a clinically applicable method for detecting CTLs.
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spelling pubmed-85698752021-11-10 Detection of granzyme B in CD3-positive cells infiltrated in lesional skin of a dog with erythema multiforme associated with zonisamide TANAKA, Nana KANEI, Toshitaka IWATA, Munetaka KAWABE, Mifumi KAMISHINA, Hiroaki MURAKAMI, Mami SAKAI, Hiroki MAEDA, Sadatoshi J Vet Med Sci Internal Medicine In human erythema multiforme (EM), cytotoxic T lymphocytes (CTLs) play an essential role in the pathogenesis. In canine EM, immunohistochemical staining with anti-CD8 antibody using frozen sections has shown the involvement of CTLs; however, CTL infiltration has never been quantitatively analyzed. We herein quantitatively analyzed CTL infiltration by immunohistochemical staining with granzyme B and CD3 antibodies using paraffin sections of a dog with EM associated with zonisamide. The present results indicated approximately 70% of cells at the border between the epidermis and dermis consisted of CTLs. Detection of granzyme B and CD3 using paraffin sections employed in this study can be a clinically applicable method for detecting CTLs. The Japanese Society of Veterinary Science 2021-08-25 2021-10 /pmc/articles/PMC8569875/ /pubmed/34433730 http://dx.doi.org/10.1292/jvms.21-0146 Text en ©2021 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Internal Medicine
TANAKA, Nana
KANEI, Toshitaka
IWATA, Munetaka
KAWABE, Mifumi
KAMISHINA, Hiroaki
MURAKAMI, Mami
SAKAI, Hiroki
MAEDA, Sadatoshi
Detection of granzyme B in CD3-positive cells infiltrated in lesional skin of a dog with erythema multiforme associated with zonisamide
title Detection of granzyme B in CD3-positive cells infiltrated in lesional skin of a dog with erythema multiforme associated with zonisamide
title_full Detection of granzyme B in CD3-positive cells infiltrated in lesional skin of a dog with erythema multiforme associated with zonisamide
title_fullStr Detection of granzyme B in CD3-positive cells infiltrated in lesional skin of a dog with erythema multiforme associated with zonisamide
title_full_unstemmed Detection of granzyme B in CD3-positive cells infiltrated in lesional skin of a dog with erythema multiforme associated with zonisamide
title_short Detection of granzyme B in CD3-positive cells infiltrated in lesional skin of a dog with erythema multiforme associated with zonisamide
title_sort detection of granzyme b in cd3-positive cells infiltrated in lesional skin of a dog with erythema multiforme associated with zonisamide
topic Internal Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569875/
https://www.ncbi.nlm.nih.gov/pubmed/34433730
http://dx.doi.org/10.1292/jvms.21-0146
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