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Real-time observation of tetrapyrrole binding to an engineered bacterial phytochrome

Near-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes are widely used for structural and functional deep-tissue imaging in vivo. To fluoresce, NIR FPs covalently bind a chromophore, such as biliverdin IXa tetrapyrrole. The efficiency of biliverdin binding directly affec...

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Detalles Bibliográficos
Autores principales: Hontani, Yusaku, Baloban, Mikhail, Escobar, Francisco Velazquez, Jansen, Swetta A., Shcherbakova, Daria M., Weißenborn, Jörn, Kloz, Miroslav, Mroginski, Maria Andrea, Verkhusha, Vladislav V., Kennis, John T. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8570541/
https://www.ncbi.nlm.nih.gov/pubmed/34746444
http://dx.doi.org/10.1038/s42004-020-00437-3