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Efficient nested-PCR-based method development for detection and genotype identification of Acanthamoeba from a small volume of aquatic environmental sample

Acanthamoeba spp. are opportunistic human pathogens that cause granulomatous amoebic encephalitis and keratitis, and their accurate detection and enumeration in environmental samples is a challenge. In addition, information regarding the genotyping of Acanthamoeba spp. using various PCR methods is e...

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Detalles Bibliográficos
Autores principales: Hsu, Tsui-Kang, Chen, Jung-Sheng, Tsai, Hsin-Chi, Tao, Chi-Wei, Yang, Yu-Yin, Tseng, Ying-Chin, Kuo, Yi-Jie, Ji, Dar-Der, Rathod, Jagat, Hsu, Bing-Mu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8571327/
https://www.ncbi.nlm.nih.gov/pubmed/34741041
http://dx.doi.org/10.1038/s41598-021-00968-2